Arthropods prove to be valuable tools in the investigation of different types of death. One of their uses is in toxicological analysis in the absence of tissues and body fluids normally sampled for such purposes. Entomotoxicology is a relatively new emerging discipline/field in forensics that needs more research. Different areas such as bioaccumulation, insect metabolism of drugs, localization of drugs in insects, and quantitative analysis of insect evidence need more attention. One reason for this is that a drug can only be detected in larvae when the rate of absorption exceeds the rate of elimination (Introna et al. 2001), also in some cases samples of pupae and third instars larvae cannot contain concentrations of the drugs, suggesting that drugs do not bio-accumulate over the entire life-cycle of the larvae. This could indicate that toxins are eliminated from the larvae’s system over time if they are not receiving a constant supply of the toxin (Gagliano-Candela and Aventaggiato, 2001). Also, toxins contained in tissues fed upon by carrion insects, have the potential for altering the developmental patterns of the mentioned fauna and so the post-mortem interval. So, it is important to continue studying how different toxins can affect the development and other parameters of insects of forensic importance. This special edition of the Forensic Entomotoxicology journal aims to bring new knowledge and challenges for the implementation of research in this discipline.