Non-Classical Human Leukocyte Antigen-G Allelic Diversity Among North Indians

Swayam Prakash, Maneesh Kumar Misra and Suraksha Agrawal*

Non-Classical Human Leukocyte Antigen-G Allelic Diversity Among North Indians

India being a land rich of ethnic diversity with more than 2,000 ethnic groups draws attention of anthropologists and population geneticists while studying the pattern of human migration. India showcases extensive social, cultural, linguistic, and biological variation. About 60,000 years ago the South Asian peninsula served as an important corridor for the dispersal of human beings from Africa. During the course of migrations people of India must have undergone differential selective pressures. Such phenomena might reshape the existing population.

Over the period of evolution, high degree of polymorphism has been observed for human leukocyte antigens. The HLA play a key role in modulating immune responses. Classical HLA antigens have been studied in different Indian populations for clinical and diversity perspective. However, non-classical classI antigens have not been explored much in Indian subcontinent.

All individuals were of Indo-Aryan linguistic family and were at least third generation residents of Uttar Pradesh, India. Three generation pedigrees were drawn to ensure no population admixture.
Informed consent was obtained from all the individual participants included in the study prior to collection of 5 mL blood in ethylenediaminetetraacetic acid (EDTA) by venipuncture.

The HLA-G frequency data was collected for 30 populations from the database10 and also from
published literature. However, only 12 studies were included where the alleles were determined by sequencing. The frequency data so collected represented 2, 3 and 4 allele nomenclatures of HLA-G. Alleles representing all the 3 nomenclatures were considered in-order to satisfy the Hardy Weinberg Equilibrium (HWE). The reason to include different nomenclature was that, when any 1 of the 3 HLA-G nomenclatures was considered the data was not found to be in accordance with the HWE.

Anthropol Open J. 2016; 2(1): 1-9. doi: 10.17140/ANTPOJ-2-106