Model Liposomal Delivery System for Drugs and Vaccines

*Corresponding author: Farrhana Z.Firdaus, Zeinab G. Khalil, Robert J. Capon, Mariusz Skwarczynski, IstvanToth* and Waleed M. Hussein*

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original research

Abstract

Background

Liposomes have been used for drug delivery since their discovery 60-years-ago. The advantages they provide as carriers have been recognised and exploited to improve the delivery of numerous drugs and eliminate harmful side-effects. Liposomal delivery has been tested for anticancer drugs, anti-tuberculosis drugs, variety of vaccines, just to list a few.

Methods

We developed a series of liposomal formulations with the addition of cholesterol and polyethylene glycol. The uptake of these formulations by human epithelial prostate cancer (PC-3) cells and mouse macrophages was examined and analysed by flow cytometry and confocal microscopy.

Results

Among the liposomes tested, small anionic liposome vesicles (≤200 nm) prepared with egg phosphatidylglycerol as the main lipid were most effectively taken up by PC-3 cells and macrophages.

Conclusion

We produced a liposome formulation that can be used as a model system for the delivery of drugs and vaccines.

Keywords

Liposomes; Drug delivery; Vaccines delivery; Egg phosphatidylglycerol; Polyethylene glycol.