An In Vitro Estimation of Glycemic Index of White Bread and Improvement of the Dietary Fiber

*Corresponding author: Anteneh Taye*

Author Affiliations

1Department of Food Science and Technology, Hope College of Business, Science and Technology, Addis Ababa, Ethiopia
2Associate Professor of Pharmacology, Department of Pharmacology and Clinical Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia
3Associate Professor of Health and Nutrition, Ethiopian Public Health Institute, Addis Ababa, Ethiopia


Taye A, Engidawork E, Urga K. An in vitro estimation of glycemic index of white bread and improvement of the dietary fiber. Adv Food Technol Nutr Sci Open J. 2016; 2(2): 83-87. doi: 10.17140/AFTNSOJ- 2-133


©2016 Taye A. This is an open access article distributed under the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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original research


Consumers of products preferably demand minimally processed products that do not contain chemical preservatives and maintain health benefits. These encourage the use of dietary fiber (DF) to combat some metabolic disorders like diabetes and other immunological compromisings. This study indicates the potential of DF to satisfy the lowering of estimated glycemic index from improved white bread. The sources were selected from crude fiber composition of different cereals and fruits. Fruits of ripen avocado mesocarps and baobab pulps composed of total pectin composition 13%, 30% respectively and selected as good sources. Analysis of total dietary fiber (TDF), insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) were carried out according to the manufacturer megazyme total dietary fiber kit protocol and methods described in Association of Official Analytical Chemists (AOAC). Improvement of DF was observed by enhancing on white breads (WB) based on the daily requirement of soluble fibers (pectin) composition, i.e. 6 g/day. Samples of avocado improved bread (AVB) and baobab improved bread (ADB) were made by mixing in proportion of wheat flour to avocado/baobab pulps (97:3); (94:6); (88:12). Analysis of proximate composition and in vitro estimation of hydrolysis index (HI) and glycemic index (GI) were done for products to evaluate the quality and impact on blood glucose level. Significant differences were observed for predicted GI between improved bread and controls, 82.84±0.37 (WB), 80.63±0.21 (ADB), 78.50±0.30 (AVB). As a result, the reductions in GI for the improved white breads indicate the delay in release of glucose from absorption by the cell due to the viscous nature of dietary fiber component. Thus, the improved white breads with appropriate soluble dietary fiber (pectin) content, reveals the potential for the reduction of blood glucose level and can be considered as functional foods (nutraceuticals) along with its moderate level degree of likeness or sensory acceptability. In general, the study has indicated that the soluble fibres can have a potential to reduce the postprandial glucose by delaying absorption in cells.


DF: Dietary Fiber; TDF: Total Dietary Fiber; IDF: Insoluble Dietary Fiber; SDF: Soluble Dietary Fiber; AOAC: Association of Official Analytical Chemists; WB: White Breads; AVB: Avocado Improved Bread; AUC: Areas Under Curves; HI: Hydrolysis Index; IDF: International Diabetes Federation; GI: Glycemic Index.


Food industries are mainly challenged by consumerʼs demand in quality, safety and minimally processed products that do not contain chemical preservatives and maintain health benefits. These encourage the use of dietary fiber (DF) as functional food (nutraceuticals).1 Functional foods play a special role to satisfy hunger, provide the necessary nutrients, and prevent nutrition related diseases.

Dietary fiber compounds composed of non-digestible carbohydrate by endogenous enzymes and a mixture of plant carbohydrate polymers.2 The physical properties of DF in food determine the physiologic effects and potential health benefits.3 Such as, influence on glucose and fat absorption and bowel function. 4

The difference in pattern of diabetes varies in countries due to economic status and forecasted to be present in 2030 according to International Diabetes Federation (IDF).5,6 Hence, this paper has shown the significant advantage of improving white breads with soluble DF (pectin) through providing an important information on a) using of agricultural sources for therapeutic and to alleviate nutrient related health problems, b) aware the value of waste disposal of agricultural or fiber sources, such as bran or peel of fruits; c) increasing the advantage of using soluble dietary fiber SDF (pectin) as raw materials in food industries.7,8

Results and Discussion

Compositions of crude fibre were screened from cereals (teff white (TW), barely without bran white (BWB), wheat whole white (WWW), wheat whole black (WWB) barely whole white (BWW)) and fruits (avocado pulp (AVP), baobab pulp (ADP)) as a source (Table 1). However, baobab pulp (Adansonia Digitata) was found higher in crude fiber content than avocado and cereals, avocado was selected among others in consideration to the economic feasibility and accessibility.

Table 1: Screening of cereals and fruits for crude fibers content in wet and dry base.


Figure 1: Extracted pectin from (a) Baobab pulp, (b) Avocado pulp.


The yield of extracted pectin from baobab pulp (a) and avocado pulp (b) (Figure 1) were found 20.94±2.42% and 3.01±1.41% respectively on dry base. The reported baobab pectin yield was obtained more than 10%.12

The yield (%) of dietary fibres TDF, IDF, SDF (p<0.05) for avocado and baobab pulp (Table 2) suggested baobab has been considered as a potential source for pectin.

Table 2: Dietary fiber composition of raw materials, avocado and baobab.


The nutrient compositions, which indicate the food quality, was indicated by its proximate composition (Table 3). The improved bread with avocado (AVB) indicated higher energy value due its fat content however it reduced the carbohydrate composition lesser by improved bread by baobab (ADB).

Table 3: Proximate value in dry base [g/100 g] for wheat flour, raw fruits and improved breads.

The intensity of releasing glucose concentration was calculated from the calibration curve using Beer’s law.11 The samples are prepared from improved white bread (product) proportionally on the basis of pectin potential at 3 levels.

Figure 2: Kinetic response of improved products.


The kinetic response (release) of glucose concentration (Figure 2) was found in decreasing the intensity from maltose (reference control) to AVB type 3 with increasing time. As a result, the improved products indicate the delay in hydrolysis time for which accounts for water retaining capacity. The HI was calculated as the relation between the AUC for a food and the AUC for a reference food, white bread, expressed as a percentage (Table 4). HI is a good predictor of glycemic response. Thus, the predicted GI was estimated using GI=39:71+(0.549*HI).13

These findings for estimation of in vitro analysis was compared with the report from Daou et al, Goni et al and Lee et al.11,14,15 The physical properties (structure, particle size, porosity and density) and chemical composition have an important effect on the soluble fractions due the water holding capacity of dietary fibres. As reported by Lecumberri et al,16 soluble fibres like pectin have a high hydration capacity, holding water and swelling to form viscous solutions (Figure 3). Thus, it adsorbs and retains other substances like glucose, minerals, no polar molecules (i.e. fats, bile acids), etc.

Table 4: Hydrolysis index and predicted GI for the samples and controls.

Figure 3: White bread (WFB), avocado improved bread (AVB), baobab improved bread (ADB).


The sensory analysis for products were described by semi-trained analysts by whom the data was analyzed in SPSS- 20 for different attributes. The result was indicated (Table 5) for the 3 products.


Table 5: Overall acceptance of the sensory attributes between products.


The degree of likeness for color and texture, there is no significant difference (p>0.05) between ADB and AVB, while WB indicates significantly different (p<0.05) from ADB and AVB. Declining the magnitudes of the degree of likeness for flavor and taste, all products (ADB, AVB and WB) have shown that there were significant differences among them. This may be from the habituated nature or alteration of users from normal circumstance unenhanced (unimproved) dietary fiber and this probably required longtime adaptation. The texture for AVB is significantly different from WB while insignificantly different from ADB. Probably, this may be from the moisture content of avocado and baobab and due to the water absorption or holding capacity of the product.


The study has argued for a hypothesis that improved white bread has no effect on estimation of GI. For this reason, analysis for estimation of hydrolysis, GI and sensory attribute was carried out for improved white bread with dietary fibre.

• As a result, the prediction for low GI in enhanced white bread was significantly differing from white bread. Hence, the present study is concluded as: The components of dietary fibre composition of selected avocado and baobab fruits pulp were found as an advantage to be integrated in a white bread.
• The potential composition of functional fibre (pectin) component in fruits indicates its physiological metabolic benefit as a nutraceuticals model.
• The dietary fibre (functional fibre) enhanced white bread suggested an apparent delay in releasing glucose concentration during hydrolysis and effects in slowing down the carbohydrate absorption in the management of blood glucose level.
• The overall acceptability for the improved white bread was liked moderately while for white bread was liked very much, however an adaptation to typical product natures interferes.


Metabolic effect of enhancing dietary fiber in human subject has not been studied and an in vivo glycemic index or blood glucose level shall be conducted for future study.
 Purification and characterization of the soluble dietary component, physico-chemical characteristics and other most related parameters like available or resistance starch were not included in the study. Hence, the measurements of such parameters are suggested.
 Consumers shall use dietary fiber enhanced foods for the management of blood glucose.
 The findings of this study can be used as base line information or an indicator to food processors and related users.



AAU-Center for Food Science and Nutrition, Megazyme Co. Ltd (Dr. Barry) – Ierland, Eng. Wudeneh, Dr. Endale, Dr. Yehune, Dr. Kaleab/Dr. Ashagrie/Dr. Dawd.

Conflicts of interest

The authors declare that they have no conflicts of interest.