Yong Qian, PhD


Biologist, Group Leader
Department of Pathology and Physiology
Research Branch
Health Effects Laboratory Division
National Institute for Occupational Safety and Health (NIOSH) 1095
Willowdale Road
Morgantown, WV 26505, USA

E-mail: yaq2@cdc.gov

Biography

Dr. Qian is a Toxicologist and a Group Leader at National Institute for Occupational Safety and Health (NIOSH), Centers for Disease Control and Prevention (CDC). He got his MD at Shanghai Second Medical University in 1985 and his PhD at West Virginia University in 1999. He was a post PhD fellow at West Virginia University from 2000-2001. From 2001-2006, he was an Associate Service Fellow at NIOSH. He has served as a group leader and a principal investigator since 2007. He has experience and expertise in molecular toxicology and cell biology. He has published more than50 peer-reviewed journal papers. His contributions to sciences are: 1) identification of novel biomarkers and molecular mechanisms of multi-walled carbon nanotube (MWCNT)-induced pulmonary diseases, including fibrosis, for early detection and treatment interventions; 2) application of bioinformatics in toxicity study and cancer research; 3) identification of toxicological mechanisms involved in printer-emitted engineered nanoparticle-induced toxicity; and 4) identification of the role of ROS and cellular cytoskeleton in heavy metal, ethanol, and perfluorooctane sulphonate in cellular toxicity.

Research Interest

His research interest includes: Nanoparticle-Induced Pulmonary Diseases, Identification of Lung Cancer Biomarkers, Perfluorinated Chemicals In Occupational Safety and Health; And Heavy Metal-Induced Carcinogenesis.

Scientific Activities

HONORS AND AWARDS

• Young Investigator Award Recipient, American Association for Cancer Research, 1997
• Young Investigator Award Recipient, American Association for Cancer Research, 1998
• Best of Overall Poster. 14th Annual A-E SOT Spring Meeting. May 3, 2002, Morgantown, WV
• CDC Nominations for 2007 Charles C. Shepard Awards. CDC, 2007

Publications

1. Qian Y, Li S. The role of specific Ig-E in respiratory syncytial virus infectious asthma. Journal of Shanghai Immunology.1990.
2. Qian Y, Li S. The role of basophile cells in respiratory syncytial virus infectious asthma. Journal of Clinical Pediatrics. 1990
3. Qian Y, Li S, Qi J. Relationship between hypersensitivity Type I and wheezing with respiratory syncytial virus (RSV) infection. The Journal of Shanghai Second. Medical University 1990; 4(2): 26-31.
4. Guappone AC, Qian Y, Weimer T, Flynn DC. An in vivo system for analysis of stable complex formation between Src and AFAP-110. Methods in Cell Science. 1996; 18: 1-11. doi: 10.1007/BF00123524
5. Qian Y, Baisden JM, Westin EH, Guappone AC, Koay TC, Flynn DC. Src can regulate carboxy terminal interactions with AFAP-110, which influence self-association, cell localization and actin filament integrity. Oncogene. 1998; 16: 2185-2195.
6. Qian Y, Guappone AC, Baisden JM, Hill W, Summy JM, Flynn DC. Monoclonal antibodies directed against AFAP-110 recognize species-specific and conserved epitopes. Hybridoma. 1999; 18: 167-175.
7. Qian Y, Baisden JM, Zot HG, W. Barry Van Winkle and Flynn DC. The carboxy terminus of AFAP-110 modulates interactions with actin filaments and regulates its ability to alter actin filament integrity and induce lamellipodia formation. Exp Cell Res. 2000; 255(1): 102-113.
8. Qian Y, Jiang BH, Leonard SS, et al. Cr(VI) causes the increase of tyrosine phosphorylation through reactive oxygen species-mediated reactions. Mol. Cell.Biochem. 2001; 222(1-2): 199-204.
9. Flynn DC, Qian Y, Baisden JM, Zot HG. AFAP-110 bridges signaling proteins to actin filaments and has an intrinsic capability to alter actin filament integrity (review). Oncogene. 2001; 20(44): 6435-6447.
10. Qian Y, Baisden JM, Cherezova L, et al. PKC phosphorylation increases the ability of AFAP-110 to cross-link actin filaments. Mol. Biol. Cell. 2002; 13(7): 2311-2322. doi: 10.1091/mbc.E01-12-0148
11. ClumpDA, Clem R, Qian Y, Guappone-Koay A, Berrebi AS, Flynn DC. The protein expression levels of the Src activating protein AFAP are developmentally regulated in brain. J. Neurobiology. 2003; 54(3): 473-85.
12. Yong Qian*, Luo J, Leonard SS, Harris GK, Flynn DC and Shi X. Ethanol-induced in vitro angiogenesis requires CDC42 activation, which involves hydrogen peroxide formation and actin filament reorganization. J. Biological Chemistry. 2003; 278(18): 16189-16197.
13. Luo J, Sun Y, Lin H, et al. Activation of JNK by vanadate induces a Fas-associated death domain (FADD) dependent death of cerebellar granule progenitors in vitro. J. Biological Chemistry. 2003; 278(7): 4542-51.
14. Summy JM, Qian Y, Jiang BH, et al. The c-Yes amino terminal SH4 and unique domains prevent actin filament rearrangement and phosphatidylinositol-3-Kinase activation by Src527F/c-Yes chimeric proteins. J. Cell Science. 2003; 116: 2585-2598.
15. Yong Qian*, Castranova V, Shi X. New perspectives in arsenic-induced cell signal transduction. Invited Review. J. Inorganic Biochemistry. . 2003; 96(2-3): 271-278.
16. Qian Y1, Corum L, Meng Q, et al. PI3K induced actin filament remodeling through Akt and p70S6K1: implication of essential role in cell migration. American J. Physiology: Cell Physiology. . 2004; 286(1): C153-63.
17. Qian Y, Gatesman AS, Baisden JM, et al. Analysis of the role of the leucine zipper motif in regulating the ability of AFAP-110 to alter actin filament integrity. J. Cellular Biochemistry. 2004; 91(3): 602-620.
18. Qian Y, Zhong X, Flynn DC, et al. ILK mediates actin filament rearrangements and cell migration and invasion througPI3K/AKT/Rac1 signaling. Oncogene. 2005; 24(19): 3154-3165
19. Qian Y, Liu KJ, Chen Y, Flynn DC, Castranova V, Shi X. Cdc42 regulates arsenic-induced NADPH oxidase activation and cell migration through actin filament reorganization. J. Biological Chemistry. 2005; 280(5): 3875-3884
20. Lin L, Qian Y, Shi X, Chen Y. Induction of a cell stress response gene RTP801 by DNA damaging agent methylmethane sulfonate through CCAAT/Enhancer binding protein. Biochemistry. 2005; 44(10): 3909-3914.
21. Feng R, Lu Y, Bowman LL, Yong Qian, Castranova V and Ding M. Inhibition of activator protein-1, NF-kappaB, and MAPKs and induction of phase 2 detoxifying enzyme activity by chlorogenic acid. J. Biological Chemistry. 2005; 280(30): 27888-95.
22. Harris GK, Qian Y, Leonard SS, Sbarra DC, Shi X. Luteolin and Chrysin Differentially Inhibit Cyclooxygenase-2 and Prostaglandin E2 Synthase Expression but similarly inhibit prostaglandin-E2 formation in RAW 264.7 cells. J. Nutrition. 2006; 136(6): 1517-21.
23. Guo L, Ma Y, Ward R, Castranova V, Shi X, Qian Y. Constructing molecular classifiers for accurate prognosis of lung adenocarcinoma. Clinical Cancer Research. 2006; 12(11): 3344-3354.
24. Ma Y, Ding Y, Qian Y, Sh Xi, Castranova V, Guo L. Predicting drug response by proteomic profiling. Clinical Cancer Research. 2006; 12(15): 4583-9
25. Ding M, Feng R, Wang SY, et al. Cyanidin-3-glucoside, a natural product derived from blackberry, exhibits chemopreventive and chemotherapeutic activity. J. Biological Chemistry. 2006; 281(25): 17359-68.
26. Guo L, Abraham J, Flynn DC, Castranova V, Shi X, and Yong Qian*. Individualized survival and treatment response predictions for breast cancers using phospho- EGFR, phospho-ER, phospho-Her2/neu, phospho-IGF-IR/In, phospho-MAPK, and phosphor p70S6K proteins. The international Journal of Biological Markers. 2007; 22(1): 1-11.
27. Ma Y, Yong Qian, Wei L, et al. Population-based molecular prognosis of breast cancer by transcriptional profiling. Clinical Cancer Research. 2007; 13(7): 2014-2022.
28. Guo L, Abraham J, Flynn DC, Castranova V, Shi X, Qian Y. A Comprehensive profile for individualized survival and treatment response predictions in patients with breast cancer: involvements of phospho-EGFR and phospho-Her2/neu proteins. The Open Clinical Cancer Journal. 2008; 2: 18-31.doi: 10.2174/1874189400802010018
29. Guo NL, Wan YW, Tosun K, et al. Confirmation of gene expression-based prediction of survival in non-small cell lung cance. r Clinical Cancer Research. 2008; 14(24): 8213-20. doi: 10.1158/1078-0432.CCR-08-0095.
30. Ding Z, Ma Y, Qian Y, Shi X, Castranova V, Guo L. An integrative genomic and proteomic approach to chemosensitivity prediction. International Journal of Oncology. 2009; 34(1): 107-15.
31. Apopa PL, Yong Qian*, Guo NL, et al. Iron nanoparticles induce human microvascular endothelial cell permeability through reactive oxygen species production and microtubule remodeling Particle and Fibre. Toxicology. 2009; 6: 1-14.doi: 10.1186/1743-8977-6-1
32. Rathnagiriswaran S, Wan YW, Abraham J, Castranova V, Qian Y, Guo NL. A population-based gene signature is predictive of breast cancer survival and chemoresponse. International Journal of Oncology. 2010; 36(3): 607-616
33. Qian Y, Ducatman A, Ward R, et al. Perfluorooctane sulphonate (PFOS) induces reactive oxygen species (ROS) production in human microvascular endothelial cells: role in endothelial permeability. Journal of Toxicology and Environmental Health. Part A 2010; 73: 819-836 doi: 10.1080/15287391003689317.
34. Wan YW, Yong Qian, Rathnagiriswaran S, Castranova V, Guo NL. A breast cancer prognostic signature predicts clinical outcomes in multiple tumor types. Oncol Rep. 2010; 24(2): 489-94.
35. Wan Y, Sabbagh E, Raese RA, et al. Hybrid models identified a 12-gene signature for lung cancer prognosis and chemoresponse Prediction. PLoS ONE. 2010; 5(8)1-15. doi: 10.1371/journal.pone.0012222
36. Jin C, Sun Y,Islam A, Yong Qian, Ducatman A. Perfluoroalkyl acids including perfluorooctane sulfonate and perfluorohexane sulfonate in firefighters. Journal of Occupational and Environmental Medicine. 2011; 53(3): 324-328. doi: 10.1097/JOM.0b013e31820d1314.
37. Snyder B, Cho Y, Qian Y, Coad J, Flynn DC, Cunnick J. AFAP1L1 is a novel adaptor protein of the AFAP family that interacts with cortactin and localizes to invadosomes. European Journal of Cell Biology. 2011; 90(5): 376-389. doi: 10.1016/j.ejcb.2010.11.016
38. Pacurari M, Yong Qian*, Porter DW, Wolfarth M, Wan Y, Luo D, Ding M, Castranova V, GuoNL. 2011 Multi-walled carbon nanotube-induced gene expression in the mouse lung: association with lung pathology. Toxicology and Applied Pharmacology. 255(1): 18-31 doi: 10.1016/j.taap.2011.05.012
39. Pacurari M, Yong Qian*, Fu W, et al. Cell permeability, migration, and reactive oxygen species induced by multi-walled carbon nanotubes in human microvascular endothelial cells. Journal of Toxicology and Environmental Health. 2012; 75(2): 112-28 doi: 10.1080/15287394.2011.615110
40. Guo NL, Wan YY, Denvir J, et al. Multi-walled carbon nanotube-induced gene signatures in the mouse lung: potential predictive value for human lung cancer risk and prognosis. Journal of Toxicology and Environmental Health, . 2012; 75(18): 1129-1153 doi: 10.1080/15287394.2012.699852.
41. Wan YY, Raese RA, Fortney JE, et al. A smoking-associated 7-gene signature for lung cancer diagnosis and prognosis. International Journal of Oncology. 2012; 41(4): 1387-1396. doi: 10.3892/ijo.2012.1556.
42. Snyder-Talkington BN, Qian Y, Castranova V, Guo NL. New perspectives for in vitro risk assessment of multi-walled carbon nanotubes: application of coculture and bioinformatics. Journal of Toxicology and Environmental Health. 2013; 15: 468-492doi: 10.1080/10937404.2012.736856.
43. Pacurari1 M, Addison J, Bondalapati N, et al. The microRNA-200 family regulates non-small cell lung cancer prognostic markers in H1299. Cells International Journal of Oncology. 2013; 43: 548-560. doi: 10.3892/ijo.2013.1963.
44. Snyder-Talkington BN, Pacurari M, Dong C, et al. Systematic analysis of multiwalled carbon nanotube-induced cellular signaling and gene expression in human small airway epithelial cells. Toxicological Sciences. 2013 ; 133: 79-89 doi: 10.1093/toxsci/kft019.
45. Snyder-Talkington BN, Schwegler-Berry D, Castranova V, Qian Y, Guo NL. Multi-walled carbon nanotubes induce human microvascular endothelial cellular effects in an alveolar-capillary co-culture with small airway epithelial cells. Particle and Fibre Toxicology. 2013; 10(1): 35. doi: 10.1186/1743-8977-10-35.
46. Snyder-Talkington BN, Dymacek D, Porter DW, et al. System-based identification of toxicity Pathways associated with multi-walled carbon nanotube-induced pathological response Toxicology and Applied Pharmacology. 2013; 272: 476-489. doi: 10.1016/j.taap.2013.06.026
47. Sisler JD, Pirela S, Friend S, et al. Small airway epithelial cells exposure to printer emitted nanoparticles induces cellular effects on human microvascular endothelial cells in an alveolar-capillary co-culture model. Nanotoxicology. 2014; 11: 1-11doi: 10.3109/17435390.2014.976603
48. Talkington BN, Dong C, Zhao X, et al. Multi-walled carbon nanotube-induced gene expression in vitro: Concordance with in vivo studies. Toxicology. 2015; 328: 66-74. doi: 10.1016/j.tox.2014.12.012
49. Dymacek J, Talkington BN, Porter DW, et al. mRNA and miRNA regulatory networks reflective of multi-walled carbon nanotube-induced lung inflammatory and fibrotic pathologies in mice. Toxicol Sci. 2015; 144(1): 51-64. doi: 10.1093/toxsci/kfu262
50. Mihalchik AL, Ding W, Porter DW, et al. Effects of nitrogen-doped multi-walled carbon nanotubes compared to pristine multi-walled carbon nanotubes on human small airway epithelial cells . Toxicology. 2015; 333: 25-36. doi: 10.1016/j.tox.2015.03.008