Ramani Ramchandran, PhD


Professor
CRI Developmental Vascular Biology Program
Translational Biomedical Research Center
Medical College of Wisconsin
Department of Pediatrics
8701 Watertown Plank Road
P.O. Box 26509, Milwaukee, WI 53226, USA

Biography

Dr. Ramchandran received his PhD from the Georgia Regents University, Augusta, Georgia, USA in 1997. Currently, he is a tenured Professor at the Medical College of Wisconsin, Milwaukee, WI. He is the Patrick J. and Margaret G. McMahon Professor in Obstetrics and Gynecology, and Vice Chair for Research in the Department of Obstetrics and Gynecology, in addition to his responsibilities as the Director of the Developmental Vascular Biology Program, and Director of the Zebrafish Drug Screening Core. His research has included investigation of the basic mechanisms of blood vessel formation in vertebrates, and how those vessels contribute to diseases. His lab studies developing zebrafish and mouse embryos to gain insights into vascular conditions associated with children, such as hemangiomas and solid tumors. In addition to studying blood vessel formation, his lab is also developing tools for performing drug screens using zebrafish embryos, which will identify targets and potential drug leads for treating pediatric vascular conditions.
Based on this research and fellowship training he/she has received several awards and honors. Some of them include the 2002 NCI Scholar Award, and the 2013 Distinguished Alumnus Award at Georgia Regents University. He serves on the editorial board of Vascular Cell, Indian Journal of Clinical Medicine and PLoS ONE journals, and is an expert reviewer for several leading journals including Cancer Research, FASEB, Journal of Biological Chemistry, Nature, Blood, PNAS, Circulation Research, and Nature Communications. He has authored 49 research articles/books, and is a member of the American Association of Cancer Research, American Association of Anatomists, American Heart Association, the North American Vascular Biology Association and the Society for Developmental Biology.

Research Interest

His research interests include: Developmental Vascular Biology: Vasculogenesis and Angiogenesis Mechanisms in Zebrafish and Mice, Vessel and Axon Guidance, Zebrafish Chemical Biology, Translational Models of Disease, Tumor Metastasis.

Scientific Activities

AWARDS AND HONORS:

• 1990- Batliboi Scholarship Award (Bachelors)
• 1991- Batliboi Scholarship Award (Masters)
• 1992- Tata Memorial Scholarship Award (Higher studies in U.S.), Graduate Research Assistantship Award (PhD)
• 1997 Third place, Sigma XI, Named in Whos Who Among Students in American Universities and Colleges, Special Recognition Award from Student Government Association, MCG
• 2001- Ruth L. Kirchstein NRSA Award
• 2002- NCI Scholar Award
• 2004- Nominated for Outstanding Mentor Award, NCI, NIH
• 2006- Named in Madison Whos Who of business leaders and professionals,Cash Performance Award, NIH
• 2007- Two Year Complimentary Membership to American Association of Anatomist (successful nomination of a Bensley Award Recipient)
• 2010- Donald and Judith Alstadt Research Foundation Award
• 2012- Clinical & Translational Science Institute – In Recognition of Outstanding Contributions to the Master of Science Program in Clinical & Translational Science
• 2013- Distinguished Alumnus Award, Georgia Regents University, Nominated for Diversity Award, Medical College of Wisconsin
• 2014- Community of Innovators Awards – Research, Medical College of Wisconsin

MEMBERSHIPS AND PROFESSIONAL SCIENTIFIC SOCIETIES:

• Past- American Association for the Advancement of Science, American Association for Cancer Research, American Society of Gene Therapy
• Current- American Association of Anatomists, Association for Laboratory Automation, North American Vascular Biology Association, Society for Developmental Biology

Publications

1. Dhanabal M, Ramchandran R, Volk R, et al Endostatin: yeast production, mutants, and antitumor effect in renal cell carcinoma. Cancer Res. 1999; 59: 189-197.
2. Ramchandran R, Dhanabal M, Volk R, et al. Anti-angiogenic activity of Restin, NC10 domain of human Collagen XV: Comparison to Endostatin. Biochem. and Biophys. Res Comm. 1999; 255: 735-739. doi: 10.1006/bbrc.1999.0248
3. Dhanabal M, Volk R, Ramchandran R, Simons M, Sukhatme VP. Cloning, expression, and in vitro activity of human Endostatin. Biochem and Biophys Res Comm. 1999; 258: 345-352. doi: 10.1006/bbrc.1999.0595
4. Lu H, Dhanabal M, Volk R, et al. Kringle 5 causes cell cycle arrest and apoptosis of endothelial cells. Biochem and Biophys Res Comm. 1999; 258: 668-673. doi: 10.1006/bbrc.1999.0612
5. Dhanabal M, Ramchandran R, Waterman MJF, et al. Endostatin induces endothelial cell apoptosis. J Biol Chemistry. 1999; 274: 11721-11726. doi: 10.1074/jbc.274.17.11721
6. Kamphaus GD, Colorado PC, Panka DJ, et al. Canstatin: A novel matrix-derived inhibitor of angiogenesis and tumor growth. J Biol Chemistry. 2000; 275: 1209-1215. doi: 10.1074/jbc.275.2.1209
7. Ramchandran R, Bengra C, Whitney B, Lanclos K, Tuan D. A (GATA)7 motif located in the 5 boundary area of the human b-globin locus control region exhibits conditional silencer activity. Am J of Hematology 2000; 65: 14-24. doi: 10.1002/1096-8652(200009)65:1<14::AID-AJH3>3.0.CO;2-F
8. Hanna NN, Seetharam S, Mauceri HJ, et al. Antitumor interaction of short-course endostatin and ionizing radiation. Cancer J. 2000; 6: 287-293.
9. Karumanchi SA, Jha V, Ramchandran R, et al. Cell surface glypicans are low-affinity endostatin receptors. Molecular Cell. 2001; 7: 811-822. doi: http://dx.doi.org/10.1016/S1097-2765(01)00225-8
10. Hanai JJ, Gloy J, Karumanchi SA, et al. Endostatin is a potential inhibitor of Wnt signaling. J Cell Biology. 2002; 158: 529-539. doi: 10.1083/jcb.200203064
11. Hanai JJ, Dhanabal M, Karumanchi SA, et al. Endostatin causes G1 arrest of endothelial cells through inhibition of cyclin D1. J Biol Chemistry. 2002; 277: 16464-16469. doi: 10.1074/jbc.M112274200
12. Chan B, Sinha S, Cho D, Ramchandran R, Sukhatme VP. Critical roles of CD146 in zebrafish vascular development. Dev Dynamics. 2005; 232: 232-244. doi: 10.1002/dvdy.20220
13. Bedell V, Yeo SY, Park KW, et al. Roundabout4 is essential for angiogenesis in vivo. PNAS. 2005; 102: 6373-78. doi: 10.1073/pnas.0408318102
14. Kaur S, Castellone, MD, Bedell VM, Konar M, Gutkind JS, Ramchandran R. Robo4 signaling in endothelial cells imply attraction guidance mechanisms. J Biol Chemistry. 2006; 281 (16): 11347-11356. doi: 10.1074/jbc.M508853200
15. Hu G, Tang J, Zhang B, et al. HSPA12B is essential in zebrafish vascular development. J Cell Science. 2006; 119, 4117-4126.
16. Arbiser JL, Kau T, Konar M, et al. Solenopsin, the alkaloidal component of the fire ant (Solenopsis invicta), is a naturally occurring inhibitor of phosphatidylinositol-3-kinase signaling and angiogenesis. Blood. 2007. 109(2): 560-565. doi: http://dx.doi.org/10.1182/blood-2006-06-029934
17. Kanungo J, Li BS, Goswami M, Zheng YL, Ramchandran R, Pant HC. Cloning and characterization of zebrafish (Danio rerio) Cyclin dependent kinase 5. Neurosci Lett. 2007; 412 (3): 233-238. doi: 10.1016/j.neulet.2006.11.016
18. Isenberg JS, Jia Y, Field L, et al. Modulation of Angiogenesis by Dithiolethione-modified NSAIDs and Valproic Acid. Br J Pharmacol. 2007; 151(1): 63-72. doi: 10.1038/sj.bjp.0707198
19. Kaur S, Abu-Abab MS, Singla S, Yeo SY, Ramchandran R. Expression pattern for unc5b, an axon guidance gene in embryonic zebrafish development. Gene Expression. 2007; 13(6): 321-327. doi: http://dx.doi.org/10.3727/000000006781510714
20. Garnaas MK, Liu M, Marx R, et al. Syx, a novel Rho A guanine exchange factor, is essential for angiogenesis in vivo. Circulation Research. 2008; 103(7):710-716. doi: 10.1161/CIRCRESAHA.108.181388
21. Kaur S, Samant GV, Pramanik K, et al. Silencing of directional migration in robo4 knockdown endothelial cells. BMC Cell Biology. 2008; 39: 61. doi: 10.1186/1471-2121-9-61
22. Makky K, Duvnjak P, Pramanik K, Ramchandran R, Mayer AN. A whole-animal microplate assay for metabolic rate using zebrafish. Journal of Bio Molecular Screening. 2008; 13(10): 960-967. doi: 10.1177/1087057108326080
23. Pramanik K, Chun CZ, Garnaas MK, et al. Dusp-5 and Snrk-1 coordinately function during vascular development and disease. Blood. 2009; 113 (5): 1184-1191. doi: 10.1182/blood-2008-06-162180
24. Kanungo J, Zhen, Y, Amin N, Kaur S, Ramchandran R, Pant H. Specific inhibition of cyclin-dependent kinase 5 activity induces motor neuron development in vivo. Biochem and Biophys. Res. Comm. 2009; 386: 263-267. doi: 10.1016/j.bbrc.2009.06.038
25. Chun CZ, Kaur S, Samant GV, et al. Snrk-1 is involved in multiple steps of angioblast development and acts via notch signaling pathway in artery-vein specification in vertebrates. Blood. 2009; 113(5): 1192-1199. doi: 10.1182/blood-2008-06-162156
26. Jia Y, Wu S-L, Isenberg JS, et al. Thiolutin inhibits endothelial cell adhesion by perturbing Hsp27 interactions with the actin cytoskeleton. Cell Stress Chaperones. 2010; 15(2): 165-181. doi: 10.1007/s12192-009-0130-0.
27. Li K, Blum Y, Verma A, et al. A noncoding antisense RNA at the tie-1 locus regulates tie-1 transcript levels function in vivo. Blood. 2010; 115(1): 133-139. doi: 10.1182/blood-2009-09-242180
28. Verma A, Bhattacharya R, Remadevi I, et al. Ecscr promotes angioblast migration during vasculogenesis and enhances kdr sensitivity. Blood. 2010; 115(22): 4614-4622. doi: 10.1182/blood-2009-10-248856
29. Zhao B, Chun C, Liu Z, et al. Nogo-B receptor is essential for angiogenesis in vivo via Akt pathway. Blood. 2010; 116(24):5423-5433. doi: 10.1182/blood-2010-02-271577
30. Chun CZ, Remadevi I, Schupp M, et al. Fli+ etsrp+ hemato-vascular progenitor cells proliferate at the lateral plate mesoderm during vasculogenesis in zebrafish. PLoS One. 2011; 6(2): e14732. doi: 10.1371/journal.pone.0014732.
31. Lakshmikanthan S, Sobczak M, Chun CZ, et al. Rap1b regulates angiogenesis by an endothelial cell-autonomous mechanism involving integrin αvβ3-dependent VEGFR2 activation. Blood. 2011; 118(7): 2015-2026.
32. SamantGV, SchuppMO, François M, et al. Sox factors transcriptionally regulate robo4 expression in the developing vasculature in zebrafish. J Biol Chem. 2011; 286(35): 30740-30747. doi: 10.1074/jbc.M111.220665
33. Patra C, Kim J, Pramanik K, et al. Reactive oxygen species driven angiogenesis by inorganic nanorods. Nano Lett. 2011; 11(11): 4932-14938. doi: 10.1021/nl2028766
34. Wickramasekera N, Gebremedhin D, Carver K, et al. Role of dual-specificity protein phosphatase-5 (DUSP-5) in modulating myogenic response in rat cerebral arteries. Paper : JAPPL-01026-2011R3. J Appl Physiol. 2013; 114(2): 252-261. doi: 10.1152/japplphysiol.01026.2011
35. Kilari S, Remadevi I, Zhao B, et al. Endothelial cell-specific chemotaxis receptor (ECSCR) enhances vascular endothelial growth factor (VEGF) receptor-2/kinase insert domain receptor (KDR) activation and promotes proteolysis of internalized KDR. J Biol Chem. 2013; 288(15): 10265-10274. doi: 10.1074/jbc.M112.413542
36. Span EA, Goodsell DS, Ramchandran R, Franzen M, Hemran T, Sem DS. Protein structure in context: The molecular landscape of angiogenesis. Biochem Mol Biol Educ. 2013; 41(4): 213-23. doi: 10.1002/bmb.20706.
37. Leigh NR, Schupp MO, Li K, et al. Mmp17b and its putative substrate Reck are essential for neural crest migration in vivo. PLoS ONE. 2013; 8(10): e76484. doi: 10.1371/journal.pone.0076484
38. Wang L, Cossette SM, Rarick KR, et al. Astrocytes directly influence tumor cell invasion and metastasis in vivo. PLoS ONE. 2013; 8(12): e80933. doi: 10.1371/journal.pone.0080933.
39. Zhou X, Fan LX, Li K, Ramchandran R, Calvet JP, Li X. SIRT2 regulates ciliogenesis and contributes to abnormal centrosome amplification caused by loss of polycystin-1. Hum Mol Genet. 2014; 23(60): 1644-1655. doi: 10.1093/hmg/ddt556
40. Schupp MO, Waas M, Chun CZ, Ramchandran R. Transcriptional inhibition of etv2 expression is essential for embryonic cardiac development. Dev Biol. 2014; Pii: S0012-1606(14)00315-7. doi: 10.1016/j.ydbio.2014.06.019