Pradeep K. Dudeja, PhD

Professor of Physiology & Director of Research
Division of Gastroenterology & Hepatology, Dept. of Medicine, UIC
Senior Research Career Scientist, Jesse Brown VAMC
Medical Research Service (600/151)
820 South Damen Avenue
Chicago, IL 60612, USA

Biography

EDUCATION

Postgraduate Training:

Ph.D. Biochemistry
• [1978-1983] Postgraduate Institute of Medical Education and Research, Chandigarh (India)
• [1975-1978] M.S. Biochemistry, G.B. Pant University of Agriculture &Technology, Nainital (India)

Postdoctoral Training:

• [1983-1988] Research Associate, Department of (GI Physiol./Biochem.) Medicine, Section of Gastroenterology, Univ. of Chicago, Chicago, IL

ACADEMIC APPOINTMENTS

University Appointments
• Director of Research, Section of Digestive Diseases & Nutrition, [2010-present]
• Director, Intestinal Transport Group, UIC & JBVAMC [2010-present]
• Professor of Physiology in Medicine (tenured), [2002-present]
• Digestive and Liver Diseases, University of Illinois at Chicago, Chicago, IL • Associate Professor of Physiology in Medicine, [1996-2002]
• Digestive and Liver Diseases, University of Illinois at Chicago, Chicago, IL • Assistant Professor of Physiology in Medicine, [1991-1996]
• Digestive and Liver Diseases, University of Illinois at Chicago, Chicago, IL • Assistant Professor (Research), Department of Medicine, [1988-1991]
• Gastroenterology Division, Michael Reese Hospital & The University of Chicago, Pritzker, School of Medicine, Chicago, IL
• Research Associate, Gastroenterology, Michael Reese [1983-1988]
• Hospital & Medical Center, Univ. of Chicago, Chicago, IL
• Tutor, Department of Biochemistry, Postgraduate [1981-1983]
• Institute of Medical Education and Research, Chandigarh, India

Research Interest

Dr. Dudejas’ research group primarily focuses on the investigations of the molecular mechanisms of NaCl and short chain fatty acid absorption in the human intestine in health and disease. The long-term goal is to understand the cellular and molecular mechanisms underlying Pathophysiology of diarrhea associated with inflammatory bowel diseases or with bacterial infections and to develop strategies and tools for better treatment of diarrheal disorders. The approaches utilized include purified plasma membranes prepared from organ donor intestines, cell culture models and molecular biology techniques to provide an integrated in-vitro and in-vivo investigational model to examine the ion transport processes. Some specific projects include understanding mechanisms of modulation of sodium-hydrogen exchangers and chloride-bicarbonate exchangers in response to infection by an important food borne pathogen Enteropathogenic E. coli. Another project involves investigating the role of probiotics (lactobacillus species) in enhancing salt and water absorption in the mammalian intestine and mechanisms underlying these beneficial effects of probiotics. Other studies focus on understanding the role of monocarboxylate transporters in human colonic short chain fatty acid absorption. Since short chain fatty acids e.g butyrate and propionate play important roles as antineoplastic and anti-inflammatory agents as well as being the preferred nutrients for colonocytes, these ongoing studies have great significance in maintenance of epithelial integrity and disease prevention in colon. The hallmark of these studies is the direct investigation of the human intestine ion transport utilizing organ donors. These studies provide great training opportunities in the area of epithelial ion and nutrient absorption, host-microbe interactions, and approaches utilizing cell culture and animal models as well as the use of cutting edge molecular and imaging technologies.
Recently our group has also initiated studies to identify novel aspects related to gut microbiome in pathophysiological basis of inflammatory bowel diseases and to identify nanomedicine based therapeutic approaches to treat the diarrhea associated with IBD. We also have strong interest in defining the mechanisms of differentiation of intestinal stem cells into intestinal endocrine L cells as it relates to the direct effect of dietary fiber derived short chain fatty acids, which have potential in treatment of diabetes and metabolic syndrome.
Dr. Dudeja proposes to continue to work more in the direction of finding novel therapeutics for diarrheal diseases and take my work into translational arena. In addition, the role of stem cell-derived L- cells in metabolic syndrome and the utilization of nanomedicine based therapeutic approaches for diarrheal diseases and IBD are more exciting to pursue in the coming years.

Scientific Activities

SCIENTIFIC SOCIETIES (Memberships):

• American Physiological Society
• American Gastroenterological Association
• New York Academy of Sciences, New York

Role on Professional Societies:

• Member, Awards Committee, American Physiological Society, [1/1/02-12/31/04]
• Member, Gastrointestinal Research Committee, American Gastroenterology Association, • Bethesda, MD, [May 2000- May 2003].
• Chair, American Gastroenterological Association (AGA), Sponsored Research Symposia • Awards Committee of the GI Research Committee, (May 2002 – May 2003) Bethesda, MD.
• Member, American Gastroenterological Association, Abstract Selection Committee for • “Epithelial Transport” and “Signal Transduction” Subgroups [December-2002]
• Chair, Abstract Selection Committee for American Gastroenterological Association, • “Epithelial Transport” and “Signal Transduction” Subgroups [December-2003]
• Member: Steering Committee, American Physiological Society, GI & Liver Section, [July 2011]
• Chair, Nominating Committee for the Election of President of American Physiological Society, • GI & Liver Section, 2011
• Chair: Awards Committee, American Physiological Society, GI & Liver Section, [July 2012- 2015]
• Chair: AGA Abstract Review Committee for “Epithelial Junctions, Barrier Function & Wound • Repair” [December 2013]

MEMBERSHIP IN COMMITTEES/ADMINISTRATIVE RESPONSIBILITIES:

• Member, Campus Research Board, UIC, Clinical Sciences: Study Section: [7/1/00-6/30/04]
• Chairman, Safety Sub-Committee, Jesse Brown VAMC, Chicago, IL., [1992- 1997]
• Member, Safety Sub-Committee, Jesse Brown VAMC, Chicago, IL.[2002-2006].
• Chairman, Safety Sub-Committee, Jesse Brown VAMC, Chicago, IL., [2006-2008]
• Member, Board of Directors, West Side Institute for Science and Education, Chicago, IL., [1996-2008].
• Member: Space Allocation Subcommittee, Jesse Brown VAMC, Chicago, IL.[2004-2008]
• Member, Research and Development Committee, Jesse Brown VAMC, Chicago, IL., [1994-Present].
• Member, Human Investigation Committee, Jesse Brown VAMC, Chicago, IL., [1992- 1994].
• Member, Animal Care & Use Committee, Jesse Brown VAMC, Chicago, IL., [1992- 1995, 2001-2004]
• Coordinator, Stephen L. Winter Memorial Library of the Section of Digestive and Liver • Diseases, Department of Medicine, Univ. of Illinois at Chicago, [1994-present].
• Coordinator, Research Conferences of the Section of Digestive and Liver Diseases, Department • of Medicine, Univ. of Illinois at Chicago, 1995-1996 and [2001-2002, 2003-2004].
• Chair, R & D Institutional Biosafety sub-committee, [4-2011-Present]
• Member, R & D Advisory Panel (RDAP)-[4-2011-Present]
• Member, University of Illinois Senate, [2013-Present]

HONORS:

• Recipient of Servier Young Investigator Prize in Pharmacology awarded by the Institute de Researches Internationales Servier, France and Chinese Pharmacological Society, [May 2002].
• Excellent English lecture award in the Chinese Young Pharmacologists Symposium. Chinese Pharmacological Society, Jan. 2002.
• Excellent paper award in the 4th East-West International Pain Conference, Apr. 2000.
• Excellent paper in Annual Academic Conference of Chinese Academy of Military Medical Science. Jan. 2001.
• Excellent PhD Dissertation, Oct. 2000.
• Excellent paper in the Annual Academic Conference of Chinese Academy of Military Medical Science. Jan. 1999
• Employee recognition award (MD Anderson Cancer Center), May 2008
• Editor’s review of Chinese Science Bulletin: A new evidence of pain specific theory from Dr. Chen (1998, 43: 1467~1471) for her important publishing in the journal.

Publications

ORIGINAL SCIENTIFIC PUBLICATIONS

Selected Publications (from a list of 195 publications in peer reviewed journals)

Most relevant to the current application:

1. Borthakur A, Bhattacharyya S, Alrefai WA, Tobacman JK, Ramaswamy K, Dudeja PK. Platelet activating factor-induced NF-kB activation and IL-8 production in intestinal epithelial cells are Bcl10 dependent. Inflamm. Bowel Diseases. 2010; 16(4): 593-603. doi: 10.1002/ibd.21092.
2. Bhattacharyya S, Dudeja PK, Tobacman JK. Tumor necrosis factor alpha-induced inflammation is increased but apoptosis is inhibited by common food additive carrageenan. J Biol Chem. 2010; 285(50): 39511-22. doi: 10.1074/jbc.M110.159681
3. Bhattacharyya S, Borthakur A, Tyagi S, et al. B-cell CLL/lymphoma 10 (BCL10) is required for NF-kappaB production by both canonical and noncanonical pathways and for NF-kappaB-inducing kinase (NIK) phosphorylation. J Biol Chem. 2010; 285(1): 522-30. doi: 10.1074/jbc.M109.050815
4. Bhattacharyya S, Dudeja PK, Tobacman J. ROS, Hsp27, and IKK? mediate dextran sodium sulfate (DSS) activation of IkBa, NfkB and IL-8. Inflammatory Bowel Diseases. 2009; 15(5): 673-683. doi: 10.1002/ibd.20821
5. Bhattacharyya S, Dudeja PK and Tobacman J. ROS, Hsp27, and IKK? mediate dextran sodium sulfate (DSS) activation of IkBa, NfkB and IL-8. Inflammatory Bowel Diseases. 2009; 15(5): 673-683. doi: 10.1002/ibd.20821

Additional recent publications of importance to the field (in chronological order):

6. Saksena S, Gill RK, Tyagi S, et al. Involvement of c-Src and PKC? in the Inhibition of Cl-/OH- Exchange Activity in Caco-2 Cells by Serotonin. J. Biol. Chem. 2005; 280 (12): 11859-11868. doi: 10.1074/jbc.M411553200
7. Gill RK, Saksena S, Tyagi S, et al. Serotonin inhibits Na+/H+ exchange activity via 5-HT4 Receptors and Activation of PKCa in Caco-2 Cells. Gastroenterology. 2005; 128: 962-974. doi: http://dx.doi.org/10.1053/j.gastro.2005.02.011
8. Alrefai WA, Wen X, Jiang W, et al. Molecular cloning and promoter analysis of downregulated in adenoma (DRA). Am J Physiol Gastrointest Liver Physiol. 2007; 293(5): G923-34. doi: 10.1152/ajpgi.00029.2007
9. Bhattacharyya S, Gill R, Chen ML, et al. Toll-like receptor 4 mediates induction of Bcl10-NFkappaB-IL-8 inflammatory pathway by Carrageenan in human intestinal epithelial cells. J. Biol. Chem. 2008; 283(16): 10550-10558. doi: 10.1074/jbc.M708833200.
10. Tobacman J, Bhattacharyya S, Borthakur A, Dudeja PK. The carrageenan diet: not recommended. Science. 2008; 321: 1040-1041. doi: 10.1126/science.321.5892.1040d
11. Walker NM, Simpson JE, Brazill JM, et al. Role of Down-regulated in adenoma anion exchanger in HCO3- secretion across murine duodenum. Gastroenterology. 2009; 136(3): 893-901. doi: 10.1053/j.gastro.2008.11.016
12. Walker NM, Simpson JE, Yen PF, et al. Down-regulated in adenoma Cl/HCO3 exchanger couples with Na/H exchanger 3 for NaCl absorption in murine small intestine. Gastroenterology. 2008; 135(5) 1645-1653. doi: 10.1053/j.gastro.2008.07.083
13. Gill RK, Borthakur A, Hodges K, et al. Mechanism(s) underlying inhibition of intestinal apical Cl/OH exchange following infection with enteropathogenic E. coli. J. Clin. Invest. 2007; 117: 428-437. doi: 10.1172/JCI29625
14. Tobacman J, Bhattacharyya S, Borthakur A, Dudeja PK. The carrageenan diet: not recommended. Science. 2008; 321: 1040-1041. doi: 10.1126/science.321.5892.1040d
15. Liu H, Singla A, Ao M, et al. Calcitonin receptor (CTR)-mediated CFTR activation in human intestinal epithelial cells (IECs). J Cell Mol Med. 2011; 20: 1582-4934. doi: 10.1111/j.1582-4934.2011.01264.x