Joy Sarkar, PhD

Assistant Research Professor
Department of Ophthalmology and Visual Sciences
Illinois Eye and Ear Infirmary
University of Illinois
College of Medicine Research Building (COMRB)
909, South Wolcott Avenue, M/C 648
Room 1080, Chicago, IL-60612, USA

 

Biography

EDUCATION/TRAINING

• (1995), Bachelor of Science (BSc) in Microbiology University of Mumbai, Mumbai, India
• (1997), Master of Science (MSc) in Microbiology University of Mumbai, Mumbai, India
• (2003), Doctor of Philosophy (PhD) in Biochemistry HN Hospital and Research Centre, University of Mumbai, Mumbai, India

POSITIONS AND EMPLOYMENT

• (1998-2000), Applications Support Scientist, Labindia-Perkin Elmer Applied Biosystems
• (1999-2000), Visiting Lecturer, K. M. Kundanani College of Pharmacy, Mumbai, India
• (2000-2001), Examiner & Paper Setter for M.Pharm Biotechnology & Molecular Biology Course, Maharashtra University of Health Sciences, (MUHS), India
• (2003-2004), Visiting Research Scholar, Tata Institute of Fundamental Research (TIFR), Mumbai, India
• (2004-2006), Postdoctoral Fellow, Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, USA
• (2006-2008), Postdoctoral Research Associate, Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, USA
• (2008-2010), Postdoctoral Research Specialist, Department of Pediatrics, Childrens Hospital University of Illinois, College of Medicine, Chicago, USA
• (2010-2013), Postdoctoral Research Specialist, Corneal Neurobiology Lab, Department of Ophthalmology & Visual Sciences, University of Illinois, Chicago, USA
• (2013-Present), Research Assistant Professor, Corneal Neurobiology Lab, Department of Ophthalmology & Visual Sciences, University of Illinois, Chicago, USA

Research Interest

His research interests include: Understanding the molecular & cellular aspects of corneal nerve regeneration via myeloid-derived suppressor cells (MDSCs) and Innate immune mechanisms involved in dry eye disease via neutrophil extracellular traps (NETs).

Scientific Activities

AWARDS AND HONORS

• (1995), 3rd Ranked and obtained a 1st Class at the Bachelor of Science Examinations in Microbiology from Modern College, University of Mumbai, India
• (1997), 1st Class at the Master of Science Examinations in Microbiology from Birla College, University of Mumbai, India
• (2000-2002), Awarded Junior Research Fellowship by Hurkisondas Nurrotumdas Medical Research Society, Mumbai, India
(2002), Awarded Travel Grant for an Advanced Course in Modern Biology organized by TIFR (Tata Institute of Fundamental Research), CSIR (Council for Scientific and Industrial Research), DST (Department of Science & Technology), Mumbai, India
• (2002-2003), Awarded Senior Research Fellowship by Hurkisondas Nurrotumdas Medical Research Society, Mumbai, India
• (2013), Awarded Early Investigator Research Grant from the Illinois Society for the Prevention of Blindness (ISPB), Illinois, USA

PROFESSIONAL MEMBERSHIPS

• (2006-2010), Member of the American Society for Biochemistry and Molecular Biology (ASBMB)
• (2006-2010), Member of the National Postdoctoral Association (NPA)
• (2006-2013), Member of American Association for the Advancement of Science (AAAS)
• (2010-Current), Member of Association for Research in Visual Sciences and Ophthalmology (ARVO)
• (2011), Invited Speaker at FASEB-Experimental Biology International Symposium, Washington DC. MiR-21 plays a Role in Hypoxia-mediated Pulmonary Artery Smooth Muscle Cell Proliferation and Migration

Publications

SELECTED PEER-REVIEWED PUBLICATIONS

1. Tibrewal S, Ivanir Y, Sarkar J, et al. Hyper-osmolar stress induces neutrophil extracellular trap formation: implications for dry eye disease. Invest Ophthalmol Vis Sci. 2014; 55(12): 7961-7969. doi: 10.1167/iovs.14-15332.
2. Byun YS, Tibrewal S, Kim E, Yco L, Sarkar J, Ivanir Y, Liu CY, Sano CM, Jain S. Kerato- cytes derived from spheroid culture of corneal stromal cells resemble tissue-resident keratocytes. PLoS One. 2014; 9(11): e112781. doi: 10.1371/journal.pone.0112781
3. Sarkar J, Chaudhary S, Jassim SH, et al. Myeloid-Derived Suppre- ssor Cells Secrete NGF and Promote Trigeminal Ganglion Neurite Growth: Implications for Corneal Nerve Regeneration.Invest Ophthalmol Vis Sci. 2013; 54 (9): 5920-5936.
4. Tibrewal S, Sarkar J, Jassim SH, et al. Tear Fluid eDNA Abundance Assay: Diagnostic and Therapeutic Implications in Dry Eye Disease. Invest Ophthalmol Vis Sci. 2013; 54(13): 8051-8061. doi: 10.1167/iovs.13-12844
5. Sonawane S, Khanolkar V, Namavari A et al. Ocular Surface Extracellular DNA and Nuclease Activity Imbalance: A New Paradigm for Inflammation in Dry Eye Disease. Invest Ophthalmol Vis Sci. 2012; 53(13): 8253-8263. doi: 10.1167/iovs.12-10430
6. Jia Y, Viswakarma N, Crawford SE, et al. Early embryonic lethality of mice with disrupted transcription cofactor PIMT/NCOA6IP/Tgs1 gene. Mech Dev. 2012; 9-12: 85-88. doi: 10.1016/j.mod.2012.08.002.
7. Gou D, Ramchandran R, Peng X, et al. MiR-210 has an antiapoptotic effect in pulmonary artery smooth muscle cells during Hypoxia. Am J Physiol Lung Cell Mol Physiol. 2012. doi: 10.1152/ajplung.00344.2011.
8. Namavari A, Chaudhary S, Ozturk O, et al. Semaphorin 7a links nerve regeneration and inflammation in the cornea. Invest Ophthalmol Vis Sci. 2012; 53 (8): 4575-4585. doi: 10.1167/iovs.12-9760
9. Chaudhary S, Namavari A, Yco L, et al. Neurotrophins and Nerve Regeneration-Associated Genes are Expressed in the Cornea After Lamellar Flap Surgery. Cornea. 2012; 31(12): 1460-1467. doi: 10.1097/ICO.0b013e318247b60e.
10. Singh DK, Sarkar J, Raghavan A, Reddy SP, Raj JU. Hypoxia modulates the expression of leucine zipper-positive MYPT1 and its interaction with Protein kinase G and Rho kinases in pulmonary arterial smooth muscle cells. Pulm Circ. 2011; 1 (4): 487-498. doi: 10.4103/2045-8932.93548
11. Sarkar J, Chaudhary S, Namavari A, et al. Corneal neurotoxicity due to topical benzalkonium chloride. Invest Ophthalmol Vis Sci. 2012; 53(4): 1792-802. doi: 10.1167/iovs.11-8775
12. Namavari A, Chaudhary S, Chang JH, et al. Cyclosporine immunomodulation retards regeneration of surgically transected corneal nerves. Invest Ophthalmol Vis Sci. 2012; 53(2): 732-740. doi:
13. Namavari A, Chaudhary S, Sarkar J, et al. Invivo serial imaging of regenerating corneal nerves after surgical transection in transgenic thy1-YFP mice. Invest Ophthalmol Vis Sci. 2011; 52(11): 8025-8032. doi: 10.1167/iovs.11-8332.
14. Sarkar J, Gou D, Turaka P, Viktorova E, Ramchandran R, Raj JU. MicroRNA-21 plays a role in hypoxia mediated pulmonary artery smooth muscle cell proliferation & migration. Am J Physiol Lung Cell Mol Physiol. 2010; 299(6): L861-L871. doi: 10.1152/ajplung.00201.2010
15. Vluggens A, Andreoletti P, Viswakarma N, et al. Reversal of mouse Acyl-CoA oxidase 1 (ACOX1) null phenotype by human ACOX1b isoform. Lab Invest. 2010; 90(5): 696-708. doi: 10.1038/labinvest.2010.46.
16. Guo D, Sarkar J, Suino-Powell K, et al. Induction of nuclear translocation of constitutive androstane receptor by peroxisome proliferator-activated receptor α synthetic ligands in mouse liver. J Biol Chem. 2007; 282(50): 36766-36776. doi: 10.1074/jbc.M707183200
17. Xia J, Liao L, Sarkar J, et al. Redundant enhancement of mouse constitutive androstane receptor transactivation by p160 coactivator family members. Arch Biochem Biophys. 2007; 468(1): 49-57. doi: 10.1016/j.abb.2007.09.005
18. Sarkar J, Qi C, Guo D, et al. Transcription coactivator PRIP, the peroxisome proliferator-activated receptor (PPAR)-interacting protein, is redundant for the function of nuclear receptors PParalpha and CAR, the constitutive androstane receptor, in mouse liver. Gene Expr. 2007; 13(4-5): 255-269.
19. Viswakarma N, Yu S, Naik S, et al. Transcriptional regulation of Cidea, mitochondrial cell death- inducing DNA fragmentation factor alpha-like effector A, in mouse liver by peroxisome proliferator-activated receptor α and γ. J Biol Chem. 2007; 282(25): 18613-18624. doi: 10.1074/jbc.M701983200
20. Matsumoto K, Yu S, Jia Y, et al. Critical role for transcription coactivator peroxisome proliferator-activated receptor (PPAR)-binding protein/TRAP220 in liver regeneration and PPARalpha ligand-induced liver tumor development. J Biol Chem. 2007; 282 (23): 17053-17060. doi: 10.1074/jbc.M701956200
21. Guo D, Sarkar J, Ahmed MR, et al. Peroxisome proliferator-activated receptor (PPAR)-binding protein (PBP) but not PPAR-interacting protein (PRIP) is required for nuclear translocation of constitutive androstane receptor in mouse liver. Biochem Biophys Res Commun. 2006; 347(2): 485-495. doi: 10.1016/j.bbrc.2006.06.129
22. Jia Y, Guo GL, Surapureddi S, et al. Transcription coactivator peroxisome proliferator-activated receptor binding protein/mediator 1 deficiency abrogates acetaminophen hepatotoxicity. Proc Natl Acad Sci USA. 2005; 102(35): 12531-12536. doi: 10.1073/pnas.0506000102
23. Marita A, Sarkar J, Rane S. Type 2 diabetes in non-obese Indian subjects is associated with reduced leptin levels: study from Mumbai, Western India. Mol Cell Biochem. 2005; 275(1-2): 143-151.