Joseph Irudayaraj, PhD

Department of Biological Engineering
Agricultural and Biological Engineering
Deputy Director
Bindley Bioscience Center Discovery Park
Purdue University


Dr. Joseph has degrees in Agricultural Engineering, Computer Sciences, and Biological Engineering. He is a Professor of Biological Engineering in the Department of Agricultural and Biological Engineering. The Primary focus of his research is on single cell technology development for detection and quantification of epigenetic modifications, transcripts, and phosphorylation with an ultimate goal to detect cancers in its earliest possible stage. His research is multidisciplinary, and he collaborates with over 10 departments spanning 5 different colleges across Purdue. He teaches two undergraduate courses (i) Engineering Analysis for Design and (ii) Statistical Methods for Engineering and one graduate course (Biophysics and Biosensors). He serves in the Nanotechnology for medicine, biological instrumentation development, and Epigenetics review panels for the NIH, NSF, NASA and DOD among other national and international (Canada, European Union, Singapore, UK) proposal review panels. He chairs the Small Business Innovative Grants program (Biological Instrumentation division) at NIH and is a reviewer for over 12 journals and in the editorial board for 5 journals.
He is a member of the Entrepreneurship Leadership Academy, a co-leader of the Drug Discovery and Molecular Sensing Program of the Purdue Center for Cancer Research and was the Graduate Chair of the ABE program (Ranked #1 by the US News and World Report, 2009-2012). He has published over 200 refereed journal articles, over 400 conference presentations, over 50 publications in conference proceedings and book chapters in areas covering Nanotechnology and biosensors, primarily addressing diagnostics, drug delivery, and numerical analysis. He is a strong proponent of training the next generation of the student scientists/engineers in cross disciplinary areas. He is a member of American Chemical Society, Institute of Biological Engineering, Biophysical Society, and the American Society of Agricultural and Biological Engineers.

Research Interest

His research interest includes: Food pathogen sensors.

Scientific Activities


• Chair, Graduate Program of ABE (Rated #1, 2009-2012), ABE, Purdue University.
• Associate Director, Physiological Sensing Facility & Biophysics Core, Bindley Bioscience Center.
• Member: Biophysical Society; American Chemical Society; American Institute of Chemical Engineering; American Society for Agric. and Biol. Engin., Institute of Biological Engineers.
• Member, College of Engineering, Dean’s Strategic Committee, Bindley Bioscience Center Task Force, College of Agriculture Area Promotions and Tenure Committee.


• Covidien, St. Louis, MO. (Single cell assays).
• bioMerieux Inc. (Pathogen detection).
• CSO Analytical Instruments Reichert Inc., NJ. (Biosensor research).
• Dow AgroSciences (Biosensor research).
• Nestle Corp. Switzerland (Food characterization research).


• Chair, Faculty climate committee (1999-2001) – Penn State – coordination of cultural events.
• Advisor, European Students club (1999 – 2003) – Penn State [Student activities].
• Advisor Board of the International Friendship House (2003).
• Participation in minority summer program opportunity and Women in Engineering programs.


• Manuscript Reviewer for : Nature Nanotechnology, Nano Letters, Angew Chemie, ACS Nano, Physics Chemical Letters, Biomedical Optics, Analytical Chemistry, Biosensors and Bioelectronics, Lab on Chip, J. Food Science, J. Molecular Structure, Transactions of the ASABE, BioTechnology Progress, Intl. J. of Heat and Mass Transfer, Transactions of the ASME etc.
• Research grant proposal reviewer for : NIH (Nano, Molecular Technology, SBIR programs); NSF (CAREER grant, SBIR programs); DoD (Idea grant); USDA-NRI, BARD, European Agricultural Research Agency, NSERC, Canada.


University Polytechnique Valencia (Spain), Korean Food Research Institute (Korea), Flanders Research Institute for Post-harvest Tech (Belgium), Univ. of Sassari (Italy), Univ of St. Petersburgh (Russia), Tata Institute of Fundamental Res.; Center for Cellular Molecular Biology (India).



1. Ravindranath S, Mauer L, Chitrita D, Irudayaraj J. Biofunctionalized magnetic nanoparticle integrated mid-infrared pathogen sensors for food matrices. Analytical Chemistry. 2009; 81(8): 2840-2846. doi: 10.1021/ac802158y.
2. Wang C, Irudayaraj J. Multifunctional magnetic-Optical nanoparticle probes for simultaneous detection, separation, ablation of multiple pathogens. Small. 2009; 2(6):283-289. doi: 10.1002/smll.200901596.
3. Wang Y, Ravindranath S, Irudayaraj J. Separation and detection of multiple pathogens in a food matrix by SERS nano probes. Anal. & BioAnal. Chem. 2010; 399 (3): 1271-8. doi: 10.1007/s00216-010-4453-6.
4. Book B, Chen J, Irudayaraj J. Quantification of receptor targeting aptamer binding characteristics using single-molecule spectroscopy. Biotech, Bioengin. 2010; 108 (5):1222-1227. doi: 10.1002/bit.23043.
5. Cho I, Irudayaraj J. In-situ immunogold nanoparticle network-based ELISA for biosensor for highly sensitive pathogen detection. Intl J Microbiology. 2013; 164(1): 70-75. doi: 10.1016/j.ijfoodmicro.2013.02.025.
6. Cho I, Irudayaraj J. Lateral-flow immune concentration for detection of Listeria monocytogenes. Analytical Bioanal Chemistry. 2013; 405(10): 3313-3319.
7. Jae-Sung K, Ravindranath S, Aloke K, Irudayaraj J, Wereley S. Opto-electrokinetic manipulation technique for high-performance on-chip biological assay. Lab on Chip. 2012; 12(23): 4955-4959. (Cover article & top 10 citations; Featured in Nature Photonics) doi: 10.1039/c2lc40662d.
8. Stephen K, Nakatsu C, Irudayaraj J. Surface Enhanced Raman Spectroscopy (SERS) for the discrimination of Arthrobacter strains based on variations in cell surface composition. Analyst. 2012; 137: 4280-4286. doi: 10.1039/c2an35578g.
9. Mura S, Greppi G, Marongiu M, et al. FTIR nanobiosensors for Escherichia coli detection. Beilstein J. Nanot. 2012; 3: 885-92.
10. Ravindranath S, Henne T, Thompson D, Irudayaraj J. Intracellular bioreduction of Chromate-decorated Gold nanoparticles by Shewanella oneidensis MR-1 by Raman chemical imaging. ACS NANO. 2011; 5(6):4729-36.
11. Guo C, Irudayaraj J. 2011. Fluorescent Ag clusters via a Protein-directed approach as a Hg(II) ion sensor. Analytical Chemistry. 83(8):2883-9. doi: 10.1021/ac1032403.
12. Cho I-H, Bhandari P, Patil P, Irudayaraj J. Membrane filter-assisted surface enhanced Raman spectroscopy for the rapid detection of E. coli O157:H7 in ground beef. Biosensors and Bioelectronics. 2014; doi: 10.1016/j.bios.2014.08.063.
13. Craig A, Franca A, Oliveira L, Irudayaraj J, Ileleji K. Application of elastic net and infrared spectroscopy in the discrimination between defective and non-defective roasted coffees. Talanta. 2014; 128: 393-400. doi: 10.1016/j.talanta.2014.05.001.
14. Cho I-H, Mauer L, Irudayaraj J. In-situ fluorescent immunomagnetic multiplex detection of foodborne pathogens in very low numbers. Biosensors and Bioelectronics. 2014; 57: 143-148. doi: 10.1016/j.bios.2014.02.012.
15. Chen H, Weng T-W, Ticcitelli M, Cui Y, Irudayaraj J, Choi J. Understanding the Mechanical Properties of DNA Origami Tiles and Controlling the Kinetics of Their Folding and Unfolding Reconfiguration. JACS. 2014; 136(19): 6995-7005. doi: 10.1021/ja500612d.
16. Kadam U, Schulz, Irudayaraj J. Detection and quantification of alternative splice sites in Arabidopsis genes AtDCL2 and AtPTB2 with highly sensitive surface enhanced Raman spectroscopy (SERS) and gold nanoprobes. FEBS letters. 2014; 588 (9): 1637-1643. doi: 10.1016/j.febslet.2014.02.061.
17. Lee K, Lee L, Irudayaraj J. In vivo real-time quantification of intracellular mRNA using plasmon spectroscopy. Nature Nanotechnology. 2014; 9(6): 474-480.
18. Cho I, Mauer L, Irudayaraj J. In-situ fluorescent immunomagnetic detection of foodborne pathogens. Biosensors and Bioelectronics. 2014; 57:143-148. doi: 10.1016/j.bios.2014.02.012.
19. Chen Y, Damayanti N, Dunn K, Irudayaraj J. Zhou F. Diversity of two forms of DNA methylation in the brain. Frontiers in Genetics. 2014; 5(46):1-23. doi: 10.3389/fgene.2014.00046.
20. Mura – S. Greppi G, Roggero P, Irudayaraj J, et al. Functionalized gold nanoparticles for the detection of nitrates in water International. J. Environmental Science and Technology. 2014.