Charlotte M. Vines, PhD


Assistant Professor
Department of Biological Sciences
Director A-Prime-Time
Border Biomedical Research Center
University of Texas
500 W. University Ave
EL Paso, TX 79968, USA

E-mail:cvines@utep.edu

Biography

Dr. Vines earned her Bachelor’s of Arts degree in Biochemistry, Molecular and Cellular Biology from Northwestern University in Evanston, IL. She went on to earn her Master’s of Science degree from the University of Houston, Clear Lake, Tx under the direction of Dr’s Philip Tofilon and Margaret Kaschau, and her PhD from Harvard University in the Division of Medical Sciences from Dr’s Frank McKeon and Bruce Schnapp. Dr. Vines completed her post doctoral studies with Dr’s Cheryl Willman and Eric Prossnitz at the University of New Mexico in lymphocyte biology. She is currently an Assistant Professor at the University of Texas at El Paso.

Research Interest

Their laboratory studies the C-C Chemokine Receptor 7 (CCR7) and its role in regulating the adaptive immune response. Under homeostatic conditions specific populations of T cells, activated B cells or innate immune cells such as activated dendritic cells, macrophages and neutrophils, use CCR7 to promote co-localization of immune cells within secondary lymph organs. These co-localization events regulate the intensity and type of adaptive immune response generated by the host. The goal of their studies is to better understand the CCR7-regulated signaling events that control the intensity of the adaptive immune response during vaccination.

Scientific Activities

MEMBERSHIPS

• American Association of Cancer Research
• American Association of Immunologists Minorities affairs committee
• Member, American Society of Cell Biology
• Member, Autumn Immunology Conference Council
• Member, Society of Leukocyte Biology

Publications

1. Wu L, Chen X, Zhao J, et al. A novel IL-17 signaling pathway controlling keratinocyte proliferation and tumorigenesis via the TRAF4-ERK5 axis. The Journal of experimental medicine. 2015; 212: 1571-1587. doi: 10.1084/jem.20150204
2. Sestak JO, Sullivan BP, Thati S, et al. Codelivery of antigen and an immune cell adhesion inhibitor is necessary for efficacy of soluble antigen arrays in experimental autoimmune encephalomyelitis. Molecular therapy. Methods & clinical development. 2014; 1: 14008. doi: 10.1038/mtm.2014.8
3. Northrup L, Sestak JO, Sullivan BP, et al. Co-delivery of autoantigen and b7 pathway modulators suppresses experimental autoimmune encephalomyelitis. The AAPS journal. 2014; 16: 1204-1213. doi: 10.1208/s12248-014-9671-y
4. Chittasupho C, Sestak J, Shannon L, Siahaan TJ, Vines CM, Berkland C. Hyaluronic acid graft polymers displaying peptide antigen modulate dendritic cell response in vitro. Molecular pharmaceutics. 2014; 11: 367-373. doi: 10.1021/mp4003909
5. Vines CM. Phospholipase C: Advances in experimental medicine and biology. 2012; 740: 235-254.
6. Shannon LA, McBurney TM, Wells MA, et al. CCR7/CCL19 controls expression of EDG-1 in T cells. The Journal of biological chemistry. 2012; 287: 11656-11664.
7. Badawi AH, Kiptoo P, Wang WT, et al. Suppression of EAE and prevention of blood-brain barrier breakdown after vaccination with novel bifunctional peptide inhibitor. Neuropharmacology. 2012; 62: 1874-1881. doi: 10.1016/j.neuropharm.2011.12.013
8. Chittasupho C, Siahaan TJ, Vines CM, Berkland C. Autoimmune therapies targeting costimulation and emerging trends in multivalent therapeutics. Therapeutic delivery. 2011; 2: 873-889. doi: 10.4155/tde.11.60.
9. Chittasupho C, Shannon L, Siahaan TJ, Vines CM, Berkland C. Nanoparticles targeting dendritic cell surface molecules effectively block T cell conjugation and shift response. ACS nano. 2011; 5: 1693-1702. doi: 10.1021/nn102159g
10. Shannon LA, Calloway PA, Welch TP, Vines CM. CCR7/CCL21 migration on fibronectin is mediated by phospholipase Cgamma1 and ERK1/2 in primary T lymphocytes. The Journal of biological chemistry. 2010; 285: 38781-38787. doi: 10.1074/jbc.M110.152173
11. Cunningham HD, Shannon LA, Calloway PA, et al. Expression of the C-C chemokine receptor 7 mediates metastasis of breast cancer to the lymph nodes in mice. Translational oncology. 2010; 3: 354-361.
12. Byers, M. A, Calloway, P. A, Shannon, L, et al. Arrestin 3 mediates endocytosis of CCR7 following ligation of CCL19 but not CCL21. Journal of Immunology. 2008; 181: 4723-4732.
13. Key TA, Vines CM, Wagene BM, Gurevich VV, Sklar LA, Prossnitz ER. Inhibition of chemoattractant N-formyl peptide receptor trafficking by active arrestins. Traffic. 2005; 6: 87-99. doi: 10.1111/j.1600-0854.2004.00248.x
14. Xue, M, Vines, C. M, Buranda, T, et al. N-formyl peptide receptors cluster in an active raft-associated state prior to phosphorylation. The Journal of biological chemistry. 2004; 279: 45175-45184. doi: 10.1074/jbc.M407053200
15. Vines CM, Prossnitz ER. Mechanisms of G protein-coupled receptor-mediated degranulation. FEMS microbiology letters. 2004; 236: 1-6. doi: 10.1111/j.1574-6968.2004.tb09619.x
16. Revankar CM, Vines CM, Cimino DF, Prossnitz ER. Arrestins block G protein-coupled receptor-mediated apoptosis. The Journal of biological chemistry. 2004; 27: 24578-24584. doi: 10.1074/jbc.M402121200
17. Vines CM, Revankar CM, Maestas DC, et al. N-formyl peptide receptors internalize but do not recycle in the absence of arrestins. The Journal of biological chemistry. 2003; 278: 41581-41584.
18. Vines CM, Xu M, Maestas DC, Cimino DF, Prossnitz ER. Regulation of N-formyl peptidemediated degranulation by receptor phosphorylation. Journal of immunology. 2002; 169: 6760-6766.
19. Vines CM, Potter JW, Xu Y, et al. Inhibition of beta 2 integrin receptor and Syk kinase signaling in monocytes by the Src family kinase Fgr. Immunity. 2001; 15: 507-519. doi: 10.1016/S1074-7613(01)00221-7
20. Gresham HD, Dale BM, Potter JW, et al. Negative regulation of phagocytosis in murine macrophages by the Src kinase family member, Fgr. The Journal of experimental medicine. 2000; 191: 515-528.
21. Bill CA, Vines CM, Garrett KC, Yamada K, Tofilon PJ. Enhancement of the radiosensitivity of two human tumour cell lines by hexamethylene bisacetamide. British journal of cancer. 1990; 61: 563-567.
22. Tofilon PJ, Vines CM, Meyn RE, Wike J, Brock WA. Heterogeneity in radiation sensitivity within human primary tumour cell cultures as detected by the SCE assay. British journal of cancer. 1989; 59: 54-60.
23. Tofilon PJ, Vines CM, Bill CA. Enhancement of radiation-induced DNA double-strand breaks and micronuclei in human colon carcinoma cells by N-methylformamide. Radiation research. 1989; 119: 166-175.
24. Arundel CM, Vines CM, Tofilon PJ. Chromatin modifications associated with Nmethylformamide- induced radiosensitization of clone A cells. Cancer research. 1988; 48: 5669-5673.
25. Tofilon PJ, Vines CM, Milas L. The effects of N-methylformamide on artificial and spontaneous metastases from a murine hepatocarcinoma. British journal of cancer. 1987; 55: 239-243.
26. Tofilon PJ, Vines CM, Milas L. N-methylformamide-mediated enhancement of in vitro tumor cell chemosensitivity. Cancer chemotherapy and pharmacology. 1986; 17: 269-273.
27. Tofilon PJ, Vines CM, Baker FL, Deen DF, Brock WA. cis-Diamminedichloroplatinum(II)-induced sister chromatid exchange: an indicator of sensitivity and heterogeneity in primary human tumor cell cultures. Cancer research. 1986; 46: 6156-6159.
28. Tofilon PJ, Vines CM, Milas L. Enhancement of in vitro chemotherapeutic activity by dimethylsulfoxide. Clinical & experimental metastasis. 1985; 3: 141-150.
29. Bill CA, Soto OB, Vines CM. C-C Chemokine receptor seven (CCR7): coming of age in vaccines. Vaccin Res Open J. 2016; 1(1): 7-9. doi: 10.17140/VROJ-1-102