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Recently Published Articles
  • 2020, February

    original research

    Recombinant Protein D from Haemophilus influenzae Induces Mouse Bactericidal Antibodies Against Typeable and Non-Typeable Haemophilus influenzae, which Partially Protect Infant Rats Against Serotype b BacteraemiaOpen Access

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    Abstract [+]

    Aim

    To evaluate the immunogenicity of a recombinant protein D from Haemophilus Influenzae (Hi) and the functional activities of the induced protein D antibodies in a mouse model.

    Methods

    Female Balb/c mice were immunised subcutaneously with recombinant protein D in the presence or absence of adjuvants and the serum immunoglobulin G (IgG) response to protein D was assessed by ELISA. The functional activity of the immune sera was evaluated in vitro using bactericidal assay against typeable Hi serotype b (Hib) and non-typeable Hi (NTHi) clinical isolates and in vivo using an infant rat bacteraemia model and a Hib clinical isolate.

    Results

    A dose-dependent IgG response was induced in mice immunised with the recombinant protein D and this response was further increased by the adjuvants used [CPG, AlPO4 and Al(OH)3], with the latter showing the greatest effect on the antibody response. Immune sera were very effective in bactericidal assay against several Hib and NTHi clinical isolates, with a higher serum bactericidal titre against the NTHi than against the Hib isolates. This is possibly due to the lower expression of protein D on the Hib isolates used in our study, compared to the NTHi isolates. In addition, anti-protein D antibodies were partially protective in vivo infant rat bacteraemia model against a challenge with Hib Eagan strain.

    Conclusion

    Our results suggest that recombinant protein D is a good vaccine candidate against Hi and should be given in combination with other vaccine candidates to ensure complete protection against Hib and NTHi.

    Keywords

    Haemophilus influenzae (Hi); Haemophilus influenzae serotype b bacteraemia; Rats.


  • 2019, December

    original research

    Analysis of Antibiotic Resistance of the Probiotic Bacteria Found in Commercial Food ProductsOpen Access

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    Aim

    The Lactobacillus is an industrially-important group of probiotic organisms that plays an important role in human health by inhibiting harmful and pathogenic bacterial growth, boosting immune function, and increasing resistance to infection. The aim of this study was to identify the probiotic bacteria Lactobacillus based on their phenotypic features and genotypic features. This study also shows the importance of probiotic bacterium, and the effects of their antibiotic resistance to human.

    Method

    Six different brands were cultured on man, rogosa and sharpe (MRS) agar. The identity of the culture was based on the characteristics of the strains of Lactobacillus spp. which was characterized using their phenotypic features (cell morphology, Gram’s staining tests which are specific for Lactobacillus genus). The bacterial deoxyribonucleic acid (DNA) was extracted by two different methods, boiled cell method and cetyl trimethylammonium bromide (CTAB) method. Furthermore, the extracted DNA yields were compared to determine which gives the best yield. The bacterial genus was detected with using genus specific primers, specific to the Lactobacillus. All the isolates were further subjected to antibiotic resistance test using disc diffusion method against a total of 4 antibiotics (Erythromycin, Tetracycline, Vancomycin and Ampicillin) and the antibiotic resistant genes of tet(M) & erm(B), were analyzed by polymerase chain reaction (PCR).

    Results

    Five isolates out of six samples (A to E) were found to exhibit multiple resistance against some of the most commonly used antibiotics. The isolates showed resistance toward tetracycline, erythromycin & vancomycin. Besides that, the isolates displayed a low-level of resistance toward ampicillin.

    Conclusion

    This study proves that antibiotic resistance is present in different species of probiotic strains, which may pose a food safety concern.

    Keywords

    Lactobacillus; Probiotics; Antibiotics; Tetracycline; Vancomycin; Erythromycin; Ampicillin; Antibiotic resistance.


  • 2019, August

    original research

    Self-Adjuvanting Peptide Vaccines Against Cervical CancerOpen Access

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    Abstract [+]

    Background

    Cervical cancer is a common cause of cancer-related deaths in women worldwide, with a fatality rate second only to breast cancer. Human papillomaviruses (HPVs) are the main causative agents of cervical cancer, and are therefore obvious targets for vaccine development. Although two prophylactic HPV vaccines have been commercialized, therapeutic vaccines against HPVs have not been developed yet. Current vaccine technologies emphasize the power of small particles in targeting immune cells, and particles of 20-50 nm have been reported to induce optimal immune responses against a variety of pathogens and cancers.

    Methods

    We synthesized new nanoparticle-based vaccines against cervical cancer by using antigenic 8Qmin peptide epitope derived from HPV-16 E7 protein, a hydrophilic poly-(L-glutamic acid) (PGA) linker, and an 8-arm poly (tert-butyl acrylate) dendrimer-based delivery system (D8).

    Results

    Four different peptides containing 8Qmin and PGA of different lengths were successfully synthesized with high yield and purity. These were then conjugated to alkyne-functionalized D8 by copper-catalyzed alkyne-azide cycloaddition “click” reaction. The conjugates self-assembled into nanoparticles, with decreased particle size corresponding to a greater number of Glu units. The four vaccine candidates were tested in C57 black 6 (C57BL/6) mice bearing well-established (7-day-old) tumors to examine their therapeutic effects.

    Conclusion

    Interestingly, only one conjugate delayed tumor growth, and montanide adjuvanted antigen, used as a positive control, failed to demonstrate any therapeutic effect.

    Keywords

    Peptide-based subunit vaccine; Human papillomavirus; Polyglutamic acid; Therapeutic cancer vaccine; Polymer-peptide conjugate; Self-adjuvanting; Cervical cancer.


About the Journal

Vaccination Research – Open Journal (VROJ) is an online open access domain designed to facilitate vaccine administration and to safeguard the immune system for protection from various illnesses.

Vaccination is the process of introducing antigen material into a person’s system so as to develop immunity against a particular pathogen. Vaccination is the most effective way to prevent infectious diseases.

Openventio is dedicated to act as an open source for vaccination and immunization techniques, procedures and post-vaccination health care.

Aims and Scope

Vaccination – Open Journal is a peer-reviewed journal that aims to be a complete and reliable source of discoveries and publications in the form of original, articles, review articles, case reports, and new vaccine technologies introduced in this field.

VROJ covers a wide array of subjects as given below:

  • Vaccine safety protocols
  • Advances in vaccination technology
  • Vaccination development, testing and regulation
  • Clinical evaluations and outcomes
  • Adjuvant in vaccine development
  • Legal aspects of vaccination
  • Public health programs and outcomes
  • Vaccination educations and training
  • Immunization
  • Vaccine administration
  • Vaccination research: Bacterial, viral, parasitic and non-infectious diseases
  • Vaccine manufacture
  • Subunit vaccines
  • Therapeutic vaccines
  • Vaccines for neglected diseases
  • Veterinary vaccines

Openventio aims to encourage documentation among the ever growing medical community to ensure that the data gets recorded and passed on to the future generation of practitioners.

Submissions for this journal are accepted from all practitioners, students from experts in the field to novices looking forward to exploring vaccination research.

The audience of VROJ includes doctors, nurses, research assistants, local community practitioners, manufacturers, and students of various universities and general communities from different parts of the world.

The journal welcomes all types of articles such as original research, review, case-report, mini-review, editorial, short-communication, book review, opinion, commentary, letter to the editor, conference proceedings, technical report, errata, illustrations, etc.

We are open to receive your comments or corrections to improve the quality of our journal.