Associate Editor


Jennifer Kielczewski, PhD

Staff Scientist
National Eye Institute
National Institutes of Health
Biological Imaging Core (BIC)
6 Center Drive Bldg. 6 Room 206B
Bethesda, MD 20892-0606

Biography

Senior level cell biologist with immunology and physiology experience in rodent animal models of ocular inflammation and neuronal degeneration. Experienced with histological and pathological analysis of murine lymphoid and ocular tissues. Highly experienced with fluorescent imaging and microscopy techniques. Proficient with dissection of delicate tissues (retina, lymph nodes) and survival surgeries/experimental injury models (intravitreal eye injections, optic nerve crush, fluorogold brain injections, and immunization) in mice.

Research Interest

• Immunology and physiology, ocular inflammation and neuronal degeneration
• Histological and pathological analysis of murine lymphoid and ocular tissues
• Fluorescent imaging and microscopy techniques, retina, lymph nodes
• Fluorogold brain injections, and immunization in mice, survival surgeries/experimental injury models (intravitreal eye injections, optic nerve crush, Immunohistochemistry, ELISA, Westerns, Multi-Color Flow Cytometry, Laser Capture Micro dissection (LCM)
• Confocal and light microscopy, time-lapse microscopy, Fundus Imaging
• Cryopreservation of tissues, cryosectioning and vibratome sectioning
• Cell culture of primary cells (human and mouse) under sterile conditions
• Molecular biology techniques (extraction of RNA, DNA, protein, PCR, gene arrays, Nanostring, transfections)
• T cell/lymphocyte separation and sorting
• Bone marrow transplantation/generating chimeric mice

Scientific Activities

HONORS & AWARDS

• CFC NIH Director’s Art Challenge ‘The Beauty of Science’ confocal image competition winner in NEI (October 2016)
• BioLegend Young Investigator Travel Award- $500 (November 2015)
• Fellows Award for Research Excellence (FARE) Travel Award- $1000 (June 2015)
• American Association of Immunology (AAI) Trainee Poster Travel Award-$300 (May 2015)
• Best Post-DocScientific Oral Presentation at NEI Annual Focus on Fellows Workshop- $500 Travel Award (October 2013)
• American Association of Immunology (AAI) Trainee AbstractTravel Award -$750 (May 2013)
• NEI Scientific Director’s Award for Outstanding Mentoring of Summer Students (October 2012)
• Confocal cover image for the journal Circulation Research, October 2009; 105(9)
• NEI Pre-Doctoral Training Fellowship (T32) in Vision Science (2007-2009)
• Clinical & Translational Science Institute (CTSI) TL1 Pre-Doctoral Scholar Fellowship (2009-2010)
• ARVO Conference Santen Pharmaceutical Travel Abstract Grant -$700 (May 2008)


PUBLICATION RECORD

• Author on 5 manuscripts, first author
• Author on 14 manuscripts/reviews, co-author
• Author on over 20 abstracts, first or co-author
• Author on 2 book chapters

Publications

1. M Manangeeswaran, JLKielczewski, N Sen, B Xu, D Ireland, L Novak,I Mcwilliams, A St. Leger, C Eng, RR Caspi, D Verthelyi. 2017.ZIKA virus infection of the Eye causes chorioretinal lesions, posterior uveitis and cellular infiltration in preparation.
2. Mary J. Mattapallil, Rachael C. Rigden, Jennifer L. Kielczewski, Carlos R. Zarate-Blades, Anthony J. St. Leger, Phyllis B. Silver, YingyosJittayasothorn, Chi-Chao Chan, and Rachel R. Caspi. 2017. Neuroprotective effects of IL-22 during CNS inflammation. In preparation.
3. Cuiyan Tan, WambuiWandu, Anthony St. Leger, Jennifer Kielczewski, Eric F. Wawrousek, Chi-Chao Chan, and IgalGery. 2017. Unlike Th1/Th17 cells, Th2/Th9 cells selectively migrate to the limbus/conjunctiva and initiate allergy-like processes.Exp Eye Res.Under current revision.
4. Jennifer Kielczewski, ReikoHorai, and Rachel R.Caspi. 2016. Tertiary lymphoid tissue is formed in the retinas of mice thatspontaneously develop autoimmune uveitis. Journal of Immunology.196(3):1013-25.
5. Reiko Horai, Carlos R. Zárate-Bladés, Patricia Dillenburg-Pilla, Jennifer Kielczewski, Jun Chen, Phyllis B. Silver, YingyosJittayasothorn, Chi-Chao Chan,Hidehiro Yamane, Kenya Honda,and Rachel R. Caspi. 2015. Microbiota-dependent activation of an autoreactive T cell receptor provokes autoimmunity in an immunologically privileged site. Immunity. 43(2), 343-353.
6. Mary J.Mattapallil, Phyllis B. Silver, Lizette M. Cortes, Anthony St. Leger,YingyosJittayasothorn,Jennifer L. Kielczewski, James J. Moon, Chi-Chao Chan and Rachel R.Caspi. 2015. Characterization of a new epitope of IRBP that induces moderate to severe uveoretinitis in mice with H-2b haplotype. IOVS.56(9) 5439-5449.
7.Dung V. Nguyen, Sergio Li Calzi, Lynn C. Shaw, Jennifer L. Kielczewski, Hannah E. Korah, and Maria B. Grant. 2013. An ocular view of the IGF-IGFBP system. Growth Hormone & IGF Research. 23 45-52. (Review)
8. Yagna P.R. Jarajapu, Jun Cai, Yuanqing Yan, Sergio Li Calzi, Jennifer L. Kielczewski, Ping Hu, Lynn C. Shaw, Sue M. Firth, Tailoi Chan-Ling, Michael E. Boulton, Robert C. Baxter, and Maria B. Grant. 2012. Protection of Blood Retinal Barrier and Systemic Vasculature by Insulin-like Growth Factor Binding Protein-3. PLoS One. 7:7 e39398.
9. Mark S. Segal, Laura Sautina, Shiyu Li, Yan Peng Diao, Alexander I. Agoulnik, Jennifer Kielczewski, Jonathan T. McGuane, Maria B. Grant, and Kirk P. Conrad. 2012. Relaxin increases human endothelial progenitor cell NO and migration and vasculogenesis in mice. Blood. 119:2 629-36.
10.Jennifer L. Kielczewski, SergioLi Calzi, Lynn C.Shaw,JunCai, Xiaoping Qi,QuanRuan, LinWu, Li Liu, Ping Hu , Tailoi Chan-Ling, Robert N.Mames, Sue Firth,Robert C. Baxter,PatricTurowski, Julia V.Busik, Michael E.Boulton, and Maria B. Grant. 2011. Free Insulin-like Growth Factor Binding Protein-3 (IGFBP-3) reduces retinal vascular permeability in association with a reduction of Acid Sphingomyelinase (ASMase). Invest Ophthalmol Vis Sci. 52:11 8278-8286.
11.Jennifer L. Kielczewski, Ping Hu, Lynn C. Shaw, Sergio Li Calzi, Robert N.Mames, Tom Gardiner, Evan McFarland, Tailoi Chan-Ling, and Maria B. Grant. 2011. Novel protective properties of IGFBP-3 results in enhanced pericyteensheathment, reduced microglial activation, increased microglial apoptosis, and neuronal protection following ischemic retinal injury. Am J Pathol.178:4 1517-1528.
12. Sergio Li Calzi, Matthew B. Neu, Lynn C. Shaw, Jennifer L. Kielczewski, Nicanor I. Moldovan, and Maria B. Grant. 2010. EPCs and pathological angiogenesis: When good cells go bad. MicrovascRes. 79: 3 207-16. (Review)
13.Jennifer L. Kielczewski, YagnaJarajapu,Evan L. McFarland, Jun Cai,Aqeela Afzal, Sergio Li Calzi, Kyung Hee Chang, Todd Lydic, Lynn C. Shaw, Julia Busik, Jeffrey Hughes, Arturo Cardounel, Kenneth Wilson, Timothy J. Lyons, Michael E. Boulton, Robert N. Mames, Tailoi Chan-Ling and Maria B. Grant. 2009. Insulin-like growth factor binding protein-3 (IGFBP-3) mediates vascular repair by enhancing nitric oxide generation. Circ Res. 105:9 897-905.
14. Julia Busik, MariaTikhonenko, AshayBhatwadekar, Madalina Opreanu, NafissaYakubova, Sergio Caballero, Danny Player, Takahiko Nakagawa,Aqeela Afzal, Jennifer Kielczewski, Sergio Li Calzi, Sungjin Kim, Shane Duclas, Mark Segal, Dennis L. Guberski, Walter J. Esselman, Michael E. Boulton, and Maria B. Grant. 2009. Diabetic retinopathy is associated with bone marrow neuropathy and a depressed peripheral clock.JExp Med. 206:13 2897-2906.
15. NilanjanaSengupta, Sergio Caballero, Sean M Sullivan, Lung-Ji Chang, Aqeela Afzal, Sergio Li Calzi, Jennifer L Kielczewski, Sabrina Prabarakan, EA Ellis, Leni Moldovan, Nicanor I Moldovan, Michael E Boulton, and Maria B Grant. 2009. Regulation of adult hematopoietic stem cells fate for enhanced tissue-specific repair. MolTher. 17:9 1594-1604.
16. Sergio Caballero, James Swaney, Kelli Moreno, Aqeela Afzal, Jennifer Kielczewski, Glenn Stoller, Amy Cavalli, William Garland, Genevieve Hansen, Roger Sabbadini, and Maria B. Grant. 2009. Anti-Sphingosine-1-Phosphate monoclonal antibodies inhibit angiogenesis and sub-retinal fibrosis in a murine model of laser-induced choroidal neovascularization.Exp Eye Res; 88:3 367-377.
17. Hangxiu Xu, Drina D. Sta. Iglesia,Jennifer L. Kielczewski, Danielle F. Valenta, Mary E. Pease, Donald J. Zack, and Harry A. Quigley. 2007. Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes. Invest Ophthalmol Vis Sci; 48:4 1674-1682.
18. Keith R.G. Martin, Harry A. Quigley, Danielle Valenta, Jennifer Kielczewski, and Mary Ellen Pease. 2006. Optic nerve dynein motor protein distribution changes with intraocular pressure elevation in a rat model of glaucoma. Exp Eye Res; 83:2 255-262.
19.Jennifer Kielczewski, Mary Ellen Pease, and Harry Quigley. 2005. The effect of experimental glaucoma and optic nerve transection on amacrine cells in rat retina. Invest Ophthalmol VisSci; 46:9 3188-3196.
20. Michael Blair, Mary Ellen Pease, John Hammond, Danielle Valenta, Jennifer Kielczewski, Hana Levkovitch-Verbin, and Harry Quigley. 2005. Effect of glatiramer acetate on primary and secondary degeneration of retinal ganglion cells in the rat. Invest Ophthalmol VisSci; 46:3 884-890.
21. Keith R.G. Martin, Harry A. Quigley, Donald J. Zack, Hana Levkovitch-Verbin, Jennifer Kielczewski, Danielle Valenta, Lisa Baumrind, Mary Ellen Pease, Ronald L. Klein, and William H. Hauswirth. 2003. Gene therapy with brain derived neurotrophic factor as a protection: retinal ganglion cells in a ratglaucoma model. Invest Ophthalmol VisSci; 44:10 4357-4365.


BOOKCHAPTERS

1.J.L. Kielczewski, R.R.Caspi. 2016. Models of autoimmune uveitis: Old and New (Chapter 6). Essentials in Ophthalmology: Animal Models of Ophthalmology Disease: Editor Chi-Chao Chan. Springer Publishers, 85-100.
2. AV Ljubimov, LC Shaw, S LiCalzi, J Kielczewski, S Caballero,MEBoulton and MB Grant. 2010. The Vascular Stem Cell. Chapter 144. Encyclopedia of the Eye. 2nd edition.


ABSTRACTS (First Author)

J.L. Kielczewski, R. Horai, and RR Caspi. 2015. Retina-specific T cells provide neuroprotection in a model of glaucoma. American Association of Immunology.194:114.21 (Poster Presentation)
J.L. Kielczewski, R. Horai, and RR Caspi. 2015. Retina-specific T cells provide neuroprotection in mouse models of glaucoma. NIHImmunology Interest Group Workshop. Potomac, MD. (Poster Presentation)
J.L. Kielczewski, R. Horai, and RR Caspi. 2014.Tertiary lymphoid tissue is formed in the retinas of mice that spontaneously develop autoimmune uveitis and contributes to disease chronicityARVO E-Abstract.55(13):2430.(Poster Presentation)
J.L. Kielczewski, R. Horai, and RR Caspi. 2014.Presence of tertiary lymphoid tissue in the retinas of mice that spontaneously develop autoimmune uveitis.NIHImmunology Interest Group Workshop. Potomac, MD. (Poster Presentation)
J.L. Kielczewski, R. Horai, and RR Caspi. 2014.Retina-specific T cells provide neuroprotection in a model of glaucoma. Invest Ophthalmol Vis Sci; 55: ARVO E-Abstract 2430. (Poster Presentation)
J.L. Kielczewski, R. Horai, and RR Caspi. 2013. Tertiary lymphoid tissue with germinal center and T follicular helper cells in immunologically privileged retinas of mice with chronic autoimmune uveitis. 190.American Association of Immunology; (Oral & Poster Presentation P4172)
J.L. Kielczewski, R. Horai, and RR Caspi. 2012. Migration of Retina-Specific T Cells into the Eye of Optic Nerve Injured Mice.Invest Ophthalmol Vis Sci; 53: ARVO E-Abstract 4270. (Poster Presentation)
J.L. Kielczewski, L. Wu, J. Cai, X. Qi, L.C. Shaw, R. Qing, R.N. Mames, J.V. Busik, M.E. Boulton, and M.B. Grant. 2011. Insulin-like Growth Factor Binding Protein-3 (IGFBP-3) Enhances Junctional Integrity via Acid Sphingomyelinase to Maintain the Mouse Blood Retinal Barrier (BRB). Invest Ophthalmol Vis Sci; 52: ARVO E-Abstract 4847. (Poster Presentation)
J.L. Kielczewski, T, Gardiner, L. Wu, S. LiCalzi, S. Firth, R. Baxter, R.N. Mames, M.E. Boulton, J.V. Busik, and M.B. Grant. 2010. IGFBP-3 Attenuates Vascular Injury by Reducing Activation of Acid Sphingomyelinase and Retinal Vascular Permeability. Invest Ophthalmol Vis Sci; 51:ARVO E-Abstract 36. (Poster Presentation)
J.L. Kielczewski, E.L. McFarland, A. Afzal, S. LiCalzi, Y.P.R Jarajapu, J. Busik, M.E. Boulton, R.N. Mames, T. Chan-Ling, M.B. Grant. 2009. IGFBP-3 Mediates Functional Revascularization in the Retina by Enhancing Nitric Oxide Production Through Activation of the Scavenger Receptor (SR-B1). Invest Ophthalmol Vis Sci; 50: ARVO E-Abstract 1284. (Poster Presentation)
J.L. Kielczewski, A. Afzal, K. Chang, L. Shaw, R. Mames, E. McFarland, T. Chan-Ling, J. Hughes, M.B. Grant. 2008. IGFBP-3 Acts as a Hematopoietic Stem Cell Homing Factor in an Adult Neovascularization Model. Invest Ophthalmol Vis Sci; 49: ARVO E-Abstract 4389. (Poster Presentation)
J. Kielczewski, M.E. Pease and H.A. Quigley. 2005. The Effect of Experimental Glaucoma and Optic Nerve Transection on Amacrine Cells in Rat Retina. Invest Ophthalmol VisSci; 46: ARVO E-Abstract 1237-B6. (Poster Presentation)